Mouse CD28 Antibody

Catalog # Availability Size / Price Qty
MAB4831-SP
MAB4831-500
MAB4831-100
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse CD28 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD28 in direct ELISAs and Western blots. Does not cross-react with recombinant human CD28, recombinant mouse (rm) CTLA-4, rmICOS, or rmPD-1.
Source
Monoclonal Rat IgG2C Clone # 61109
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CD28
Lys21-Lys149
Accession # P31041
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD28 Fc Chimera (Catalog # 483-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD28

CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7‑1 and B7‑2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up‑regulation of Bcl‑xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation. Agonist activity has been reported using MAB4831 (4,5).

References
  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.
  4. Nguyen, P. et al. (2003) Blood 13:4320.
  5. Orbach, A. et al. (2007) J. Immunol. 179:7287.
Entrez Gene IDs
940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)
Alternate Names
CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

Product Datasheets

Citations for Mouse CD28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. T cells specific for post-translational modifications escape intrathymic tolerance induction
    Authors: B Raposo, P Merky, C Lundqvist, H Yamada, V Urbonavici, C Niaudet, J Viljanen, J Kihlberg, B Kyewski, O Ekwall, R Holmdahl, J Bäcklund
    Nat Commun, 2018;9(1):353.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Transcriptional regulator CTR9 inhibits Th17 differentiation via repression of IL-17 expression.
    Authors: Yoo, Hyeon-Se, Choi, Yongwook, Ahn, Narae, Lee, Saseong, Kim, Wan-Uk, Jang, Min Seon, Jang, Myoung H, Kim, Yon Su, Yoo, Joo-Yeon
    J Immunol, 2014;192(4):1440-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. CD73 expression on extracellular vesicles derived from CD4+ CD25+ Foxp3+ T cells contributes to their regulatory function.
    Authors: Smyth L, Ratnasothy K, Tsang J, Boardman D, Warley A, Lechler R, Lombardi G
    Eur J Immunol, 2013;43(9):2430-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse CD28 Antibody

There are currently no reviews for this product. Be the first to review Mouse CD28 Antibody and earn rewards!

Have you used Mouse CD28 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review