Mouse CD28 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (4)
Supplemental Products

Mouse CD28 Antibody Summary

Species Reactivity
Detects mouse CD28 in direct ELISAs and Western blots. Does not cross-react with recombinant human CD28, recombinant mouse (rm) CTLA-4, rmICOS, or rmPD-1.
Monoclonal Rat IgG2C Clone # 61109
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CD28
Accession # P31041
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse CD28 Fc Chimera (Catalog # 483-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD28

CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7‑1 and B7‑2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up‑regulation of Bcl‑xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation. Agonist activity has been reported using MAB4831 (4,5).

  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.
  4. Nguyen, P. et al. (2003) Blood 13:4320.
  5. Orbach, A. et al. (2007) J. Immunol. 179:7287.
Entrez Gene IDs
940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)
Alternate Names
CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

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Citations for Mouse CD28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. T cells specific for post-translational modifications escape intrathymic tolerance induction
    Authors: B Raposo, P Merky, C Lundqvist, H Yamada, V Urbonavici, C Niaudet, J Viljanen, J Kihlberg, B Kyewski, O Ekwall, R Holmdahl, J Bäcklund
    Nat Commun, 2018-01-24;9(1):353.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Transcriptional regulator CTR9 inhibits Th17 differentiation via repression of IL-17 expression.
    Authors: Yoo, Hyeon-Se, Choi, Yongwook, Ahn, Narae, Lee, Saseong, Kim, Wan-Uk, Jang, Min Seon, Jang, Myoung H, Kim, Yon Su, Yoo, Joo-Yeon
    J Immunol, 2014-01-13;192(4):1440-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. CD73 expression on extracellular vesicles derived from CD4+ CD25+ Foxp3+ T cells contributes to their regulatory function.
    Authors: Smyth L, Ratnasothy K, Tsang J, Boardman D, Warley A, Lechler R, Lombardi G
    Eur J Immunol, 2013-07-15;43(9):2430-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function.
    Authors: Nguyen P, Moisini I, Geiger TL
    Blood, 2003-08-28;102(13):4320-5.
    Applications: Functional Assay


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