Mouse CD3 MAb (Clone 17A2)

Name: Mouse CD3 MAb (Clone 17A2)
Brand: R&D Systems
SKU: MLDP1

Catalog #: MLDP1 Datasheet / COA / SDS

Newer Version Available: MAB4841

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Discontinued Product

MLDP1 has been discontinued and is replaced by MAB4841.

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Mouse CD3 MAb (Clone 17A2) Summary

Species Reactivity

Mouse

Specificity

This antibody reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.¹

Source

Monoclonal Rat IgG2b Clone # 17A2

Label

Unconjugated

Applications

Recommended Concentration

Sample

Cell depletion

This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete CD3⁺ cells from 1 x 10⁹ bone marrow cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Cell Depletion

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Cell Depletion

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Cell Depletion

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Figure 1:Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr‑1 (Ly‑6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems, Catalog # MAB1356; dotted histogram) in bone marrow progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE