Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 10
Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 10 Summary
T Cell Enrichment Columns
|View Full Assay Principle|
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.
CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 43% and 51% of initial CD3+ cells loaded. The purity of recovered cells ranges from 83% to 90%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.
- Available for human, mouse, and rat cells
- Purifies CD3+ T cells to 83 – 90%
- Based upon gravitational flow
- Simple procedure
- No specialized equipment required
- Cell Selection Column
- 10X Column Buffer
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Citations for Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 10
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD
Authors: BW Richmond, S Mansouri, A Serezani, S Novitskiy, JB Blackburn, RH Du, H Fuseini, S Gutor, W Han, J Schaff, G Vasiukov, MK Xin, DC Newcomb, L Jin, TS Blackwell, VV Polosukhin
Mucosal Immunol, 2020;0(0):. 2020
Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis
Authors: E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn
Cancer Res., 2019;0(0):. 2019
The mechanistic study behind suppression of GVHD while retaining GVL activities by myeloid-derived suppressor cells
Authors: J Zhang, HM Chen, G Ma, Z Zhou, D Raulet, AL Rivera, SH Chen, PY Pan
Leukemia, 2019;0(0):. 2019
T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
Authors: HR Kim, Y Mun, KS Lee, YJ Park, JS Park, JH Park, BN Jeon, CH Kim, Y Jun, YM Hyun, M Kim, SM Lee, CS Park, SH Im, CD Jun
Nat Commun, 2018;9(1):3630. 2018
An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
Authors: HR Kim, HS Lee, KS Lee, ID Jung, MS Kwon, CH Kim, SM Kim, MH Yoon, YM Park, SM Lee, CD Jun
Sci Rep, 2017;7(1):8731. 2017
A novel fusion of ALT-803 (IL-15 Superagonist) with an antibody demonstrates antigen-specific antitumor responses
J Biol Chem, 2016;0(0):. 2016
CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses.
Authors: Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL
Diabetes, 2007;56(3):809-17. 2007
Ghrelin promotes thymopoiesis during aging.
Authors: Dixit VD, Yang H, Sun Y, Weeraratna AT, Youm YH, Smith RG, Taub DD
J. Clin. Invest., 2007;117(10):2778-90. 2007
CD4+ T cells from Copolymer-1 immunized mice protect dopaminergic neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
Authors: Laurie C, Reynolds A, Coskun O, Bowman E, Gendelman HE, Mosley RL
J. Neuroimmunol., 2007;183(1):60-8. 2007
IL-7 induces myelopoiesis and erythropoiesis.
Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
J. Immunol., 2007;178(3):1553-63. 2007
Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
Blood, 2007;111(1):219-28. 2007
Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis.
Authors: Onoyama I, Tsunematsu R, Matsumoto A, Kimura T, de Alboran IM, Nakayama K, Nakayama KI
J. Exp. Med., 2007;204(12):2875-88. 2007
3BP2 deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation.
Authors: de la Fuente MA, Kumar L, Lu B, Geha RS
Mol. Cell. Biol., 2006;26(14):5214-25. 2006
Cutting edge: rho activation and actin polarization are dependent on plexin-A1 in dendritic cells.
Authors: Eun SY, O'Connor BP, Wong AW, van Deventer HW, Taxman DJ, Reed W, Li P, Blum JS, McKinnon KP, Ting JP
J. Immunol., 2006;177(7):4271-5. 2006
PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes.
Authors: Hermann-Kleiter N, Thuille N, Pfeifhofer C, Gruber T, Schafer M, Zitt C, Hatzelmann A, Schudt C, Leitges M, Baier G
Blood, 2006;107(12):4841-8. 2006
Phylogenetic disparity influences the predominance of direct over indirect pathway of antigen presentation in islet xenotransplantation.
Authors: Andres A, Toso C, Morel P, Bosco D, Bucher P, Oberholzer J, Mathe Z, Mai G, Wekerle T, Berney T, Buhler LH
Transplant. Proc., 2005;37(1):463-5. 2005
Thrombospondin plays a vital role in the immune privilege of the eye.
Authors: Zamiri P, Masli S, Kitaichi N, Taylor AW, Streilein JW
Invest. Ophthalmol. Vis. Sci., 2005;46(3):908-19. 2005
SPAK kinase is a substrate and target of PKCtheta in T-cell receptor-induced AP-1 activation pathway.
Authors: Li Y, Hu J, Vita R, Sun B, Tabata H, Altman A
EMBO J., 2004;23(5):1112-22. 2004
Immunopharmacological and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpR motifs.
Authors: Wang D, Li Y, Yu D, Song SS, Kandimalla ER, Agrawal S
Int. J. Oncol., 2004;24(4):901-8. 2004
Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
J. Neurosci., 2004;24(27):6133-43. 2004
Ectopic expression of CCL19 impairs alloimmune response in mice.
Authors: Krautwald S, Ziegler E, Forster R, Ohl L, Amann K, Kunzendorf U
Immunology, 2004;112(2):301-9. 2004
Regulatory effects of osteoprotegerin on cellular and humoral immune responses.
Authors: Stolina M, Guo J, Faggioni R, Brown H, Senaldi G
Clin. Immunol., 2003;109(3):347-54. 2003
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