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Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 25

Catalog #: MTCC-525
22 Citations Datasheet / COA / SDS

Discontinued Product

MTCC-525 has been discontinued.
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R&D Systems Cell Enrichment Column Kits: Antibody-coated Silica Beads
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Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 25 Summary

T Cell Enrichment Columns

T Cell Enrichment Columns Assay Principle
View Full Assay Principle

Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.

CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 43% and 51% of initial CD3+ cells loaded. The purity of recovered cells ranges from 83% to 90%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.

Features

  • Available for human, mouse, and rat cells
  • Purifies CD3+ T cells to 83 – 90%
  • Based upon gravitational flow
  • Simple procedure
  • No specialized equipment required

Kit Contents

  • Cell Selection Column
  • 10X Column Buffer

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

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COA
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Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Citations for Mouse CD3+ T Cell Enrichment Column, Mini, Pack of 25

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD
    Authors: BW Richmond, S Mansouri, A Serezani, S Novitskiy, JB Blackburn, RH Du, H Fuseini, S Gutor, W Han, J Schaff, G Vasiukov, MK Xin, DC Newcomb, L Jin, TS Blackwell, VV Polosukhin
    Mucosal Immunol, 2020-09-23;0(0):.  2020-09-23
  2. Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis
    Authors: E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn
    Cancer Res., 2019-10-01;0(0):.  2019-10-01
  3. The mechanistic study behind suppression of GVHD while retaining GVL activities by myeloid-derived suppressor cells
    Authors: J Zhang, HM Chen, G Ma, Z Zhou, D Raulet, AL Rivera, SH Chen, PY Pan
    Leukemia, 2019-02-08;0(0):.  2019-02-08
  4. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
    Authors: HR Kim, Y Mun, KS Lee, YJ Park, JS Park, JH Park, BN Jeon, CH Kim, Y Jun, YM Hyun, M Kim, SM Lee, CS Park, SH Im, CD Jun
    Nat Commun, 2018-09-07;9(1):3630.  2018-09-07
  5. An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
    Authors: HR Kim, HS Lee, KS Lee, ID Jung, MS Kwon, CH Kim, SM Kim, MH Yoon, YM Park, SM Lee, CD Jun
    Sci Rep, 2017-08-18;7(1):8731.  2017-08-18
  6. A novel fusion of ALT-803 (IL-15 Superagonist) with an antibody demonstrates antigen-specific antitumor responses
    J Biol Chem, 2016-09-20;0(0):.  2016-09-20
  7. Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis.
    Authors: Onoyama I, Tsunematsu R, Matsumoto A, Kimura T, de Alboran IM, Nakayama K, Nakayama KI
    J. Exp. Med., 2007-11-12;204(12):2875-88.  2007-11-12
  8. Ghrelin promotes thymopoiesis during aging.
    Authors: Dixit VD, Yang H, Sun Y, Weeraratna AT, Youm YH, Smith RG, Taub DD
    J. Clin. Invest., 2007-10-01;117(10):2778-90.  2007-10-01
  9. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
    Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
    Blood, 2007-09-20;111(1):219-28.  2007-09-20
  10. CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses.
    Authors: Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL
    Diabetes, 2007-03-01;56(3):809-17.  2007-03-01
  11. IL-7 induces myelopoiesis and erythropoiesis.
    Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
    J. Immunol., 2007-02-01;178(3):1553-63.  2007-02-01
  12. CD4+ T cells from Copolymer-1 immunized mice protect dopaminergic neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
    Authors: Laurie C, Reynolds A, Coskun O, Bowman E, Gendelman HE, Mosley RL
    J. Neuroimmunol., 2006-12-28;183(1):60-8.  2006-12-28
  13. Cutting edge: rho activation and actin polarization are dependent on plexin-A1 in dendritic cells.
    Authors: Eun SY, O'Connor BP, Wong AW, van Deventer HW, Taxman DJ, Reed W, Li P, Blum JS, McKinnon KP, Ting JP
    J. Immunol., 2006-10-01;177(7):4271-5.  2006-10-01
  14. 3BP2 deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation.
    Authors: de la Fuente MA, Kumar L, Lu B, Geha RS
    Mol. Cell. Biol., 2006-07-01;26(14):5214-25.  2006-07-01
  15. PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes.
    Authors: Hermann-Kleiter N, Thuille N, Pfeifhofer C, Gruber T, Schafer M, Zitt C, Hatzelmann A, Schudt C, Leitges M, Baier G
    Blood, 2006-03-02;107(12):4841-8.  2006-03-02
  16. Thrombospondin plays a vital role in the immune privilege of the eye.
    Authors: Zamiri P, Masli S, Kitaichi N, Taylor AW, Streilein JW
    Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):908-19.  2005-03-01
  17. Phylogenetic disparity influences the predominance of direct over indirect pathway of antigen presentation in islet xenotransplantation.
    Authors: Andres A, Toso C, Morel P, Bosco D, Bucher P, Oberholzer J, Mathe Z, Mai G, Wekerle T, Berney T, Buhler LH
    Transplant. Proc., 2005-01-01;37(1):463-5.  2005-01-01
  18. Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
    Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
    J. Neurosci., 2004-07-07;24(27):6133-43.  2004-07-07
  19. Ectopic expression of CCL19 impairs alloimmune response in mice.
    Authors: Krautwald S, Ziegler E, Forster R, Ohl L, Amann K, Kunzendorf U
    Immunology, 2004-06-01;112(2):301-9.  2004-06-01
  20. Immunopharmacological and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpR motifs.
    Authors: Wang D, Li Y, Yu D, Song SS, Kandimalla ER, Agrawal S
    Int. J. Oncol., 2004-04-01;24(4):901-8.  2004-04-01
  21. SPAK kinase is a substrate and target of PKCtheta in T-cell receptor-induced AP-1 activation pathway.
    Authors: Li Y, Hu J, Vita R, Sun B, Tabata H, Altman A
    EMBO J., 2004-02-26;23(5):1112-22.  2004-02-26
  22. Regulatory effects of osteoprotegerin on cellular and humoral immune responses.
    Authors: Stolina M, Guo J, Faggioni R, Brown H, Senaldi G
    Clin. Immunol., 2003-12-01;109(3):347-54.  2003-12-01

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