Mouse CD39/ENTPD1 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD39 is observed and no cross-reactivity with recombinant mouse CD39L3 or recombinant human CD39L4 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 495826
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1
    Thr38-Ile478
    Accession # AAH11278
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD39/ENTPD1 Monoclonal Antibody (Catalog # MAB4398, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (1), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (2). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (3). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (4). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (5).

  • References:
    1. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
    2. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
    3. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
    4. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
    5. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
  • Long Name:
    Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
  • Entrez Gene IDs:
    953 (Human); 12495 (Mouse); 64519 (Rat)
  • Alternate Names:
    ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Expression and distribution of ectonucleotidases in mouse urinary bladder.
    Authors: Yu W, Robson SC, Hill WG
    PLoS ONE, 2011;6(4):e18704.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
ExactaChIP Chromatin Immunoprecipitation Kits
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ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Isotype Controls
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Rat IgG1 Isotype Control

Ctrl MAB005 24  
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Normal Rat IgG Control

Ctrl 6-001-A 5
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG1 Isotype Control

Ctrl MAB005R
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
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Rat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF005 3  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 1  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 1
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115
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