Mouse CD39L3/ENTPD3 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD39L3/ENTPD3 in direct ELISAs. In Western blots, less than 15% cross-reactivity with recombinant human (rh) CD39L4 and no cross-reactivity with rhCD39L3, recombinant mouse (rm) CD39, or rmCD39L2 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 568610
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L3/ENTPD3
    Gln44-Pro485
    Accession # NP_848791
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD39L3/ENTPD3 in Neuro-2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Mouse CD39L3/ENTPD3 Monoclonal Antibody (Catalog # MAB4464, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39L3/ENTPD3

Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3) is an integral membrane protein with an extracellular catalytic domain (1). rhNTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-3 ectodomain. NTPDase-3 hydrolyzes the beta - and gamma -phosphate groups of nucleotides, preferring ATP, ADP, UTP, and UDP as substrates (1). Through its hydrolysis of extracellular nuceotides, NTPDase-3 is important for the regulation of purinergic signaling (2). The enzyme is expressed at its highest levels in brain, pancreas, spleen, and prostate tissues (3). In the brain, NTPDase-3 may play a role in the regulation of feeding, sleep, and other behaviors (4). 

  • References:
    1. Lavoie, E.G. et al. (2004) Biochem. Pharmacol. 67:1917.
    2. Crawford, P.A. et al. (2007) Arch. Biochem. Biophys. 457:7.
    3. Chadwick, B.P. and A.M. Frischauf (1998) Genomics 50:357.
    4. Belcher, S.M. et al. (2006) Neuroscience 137:1331.
  • Long Name:
    Ecto-Nucleoside Triphosphate Diphosphohydrolase 3
  • Entrez Gene IDs:
    956 (Human); 215446 (Mouse); 316077 (Rat)
  • Alternate Names:
    ATPDase; CD39 antigen-like 3; CD39L3; CD39L3EC 3.6.1.5; CD39-like 3; EC 3.6.1; Ecto-apyrase 3; Ecto-ATP diphosphohydrolase 3; Ecto-ATPase 3; Ecto-ATPDase 3; ectonucleoside triphosphate diphosphohydrolase 3; ENTPD3; FLJ93839; HB6; HB6NTPDase 3; NTPDase-3
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 37  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG1 Isotype Control

Ctrl MAB005R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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