Mouse CD39L3/ENTPD3 Antibody

Catalog # Availability Size / Price Qty
AF4464
AF4464-SP

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Detection of Mouse CD39L3/ENTPD3 by Simple WesternTM.
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Product Details
Citations (3)
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Mouse CD39L3/ENTPD3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD39L3/ENTPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human CD39L3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L3/ENTPD3
Gln44-Pro485
Accession # AAH79871
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
Neuro-2A mouse neuroblastoma cell line, mouse salivary gland, and mouse spinal cord.
Simple Western
20 µg/mL
Neuro‑2A mouse neuroblastoma cell line
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse CD39L3/ENTPD3 (Catalog # 4464-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western View Larger

Detection of Mouse CD39L3/ENTPD3 by Simple WesternTM. Simple Western lane view shows lysates of Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD39L3/ENTPD3 at approximately 127 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4464). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Western Blot View Larger

Detection of Mouse CD39L3/ENTPD3 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, mouse salivary gland, and mouse spinal cord. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4464) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L3/ENTPD3 at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L3/ENTPD3

CD39L3 (CD39-like 3; also HB6 and ectonucleoside triphosphate diphosphohydrolase-3/eNTPDase 3) is an 80 kDa, class I member of the GDA1/CD39 NTPase family of transmembrane (TM) enzymes. It is a cell surface glycoprotein that hydrolyses both extracellular ATP and ADP. Thus, it controls ATP activation of P2X ion channel purinoreceptors. Mouse CD39L3 is 529 amino acids (aa) in length. It contains two TM segments plus an N- and C-terminal cytoplasmic region. Based on human CD39L3, the mouse extracellular domain (ECD) will contain aa 44-485. Within the ECD, mouse CD39L3 shares 82% and 94% aa identity with human and rat CD39L3 ECD, respectively.

Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 3
Entrez Gene IDs
956 (Human); 215446 (Mouse); 316077 (Rat)
Alternate Names
ATPDase; CD39 antigen-like 3; CD39L3; CD39L3EC 3.6.1.5; CD39-like 3; EC 3.6.1; Ecto-apyrase 3; Ecto-ATP diphosphohydrolase 3; Ecto-ATPase 3; Ecto-ATPDase 3; ectonucleoside triphosphate diphosphohydrolase 3; ENTPD3; FLJ93839; HB6; HB6NTPDase 3; NTPDase-3

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Citations for Mouse CD39L3/ENTPD3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Polarized ATP distribution in urothelial mucosal and serosal space is differentially regulated by stretch and ectonucleotidases
    Authors: Weiqun Yu
    American Journal of Physiology-Renal Physiology
  2. Deletion of ENTPD3 does not impair nucleotide hydrolysis in primary somatosensory neurons or spinal cord
    Authors: Eric McCoy, Sarah Street, Bonnie Taylor-Blake, Jason Yi, Martin Edwards, Mark Wightman et al.
    F1000Research
  3. Expression and distribution of ectonucleotidases in mouse urinary bladder.
    Authors: Yu W, Robson SC, Hill WG
    PLoS ONE, 2011-04-14;6(4):e18704.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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