Mouse CD5 Alexa Fluor® 405-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse CD5 in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 53-7.3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse thymus or spleen
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD5 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB115V) or (B) Rat IgG2A Alexa Fluor 405 Isotype Control (Catalog # IC006V). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

  • References:
    1. Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
    2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
    3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
    4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
    5. Luo, W. et al. (1992) J. Immunol. 148:1630.
    6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.
  • Entrez Gene IDs:
    921 (Human); 12507 (Mouse)
  • Alternate Names:
    CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 405-conjugated Antibody

Ctrl IC006V
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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