Mouse CD5 Alexa Fluor® 700-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD5 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB115N) or (B) Rat IgG2AAlexa Fluor 700 Isotype Control (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.
- Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
- Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
- van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
- Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
- Luo, W. et al. (1992) J. Immunol. 148:1630.
- Lanier, L.L. et al. (1986) J. Immunol. 137:2735.
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