Mouse CD5 Antibody

Catalog # Availability Size / Price Qty
MAB115
MAB115-SP
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Mouse CD5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD5.
Source
Monoclonal Rat IgG2A Clone # 53-7.3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus or spleen
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunohistochemistry
van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
 
Immunoprecipitation
Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
 
Blocking B Cell Proliferation
Bikah, G. et al. (1998) Int. Immunol. 10:1185.
 
Complement-dependent Cytotoxicity
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
 
CyTOF-reported
Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

References
  1. Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
  4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
  5. Luo, W. et al. (1992) J. Immunol. 148:1630.
  6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.
Entrez Gene IDs
921 (Human); 12507 (Mouse)
Alternate Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1

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