Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 53-7.3
View all CD5 Primary Antibodies »

Product Specifications

Immunogen

Mouse thymus or spleen

Specificity

Detects mouse CD5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CD5 Fluorescein‑conjugated Antibody

Detection of CD5 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB115F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD5 Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a member of scavenger receptor cysteine-rich protein superfamily (1). CD5 is a membrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is a ligand of the B cell differentiation antigen CD72.

References

  1. Ledbetter, J.A. and L.A. Herzenberg, (1979) Immunol. Rev. 47:63.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
  4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
  5. Luo, W. et al. (1992) J. Immunol. 148:1630.
  6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.

Alternate Names

CD5

Entrez Gene IDs

921 (Human); 12507 (Mouse)

Gene Symbol

CD5

Additional CD5 Products

Product Documents for Mouse CD5 Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD5 Fluorescein‑conjugated Antibody

For research use only

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