Detection of CD5 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB115F) or (B) Rat IgG2A Fluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a member of scavenger receptor cysteine-rich protein superfamily (1). CD5 is a membrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is a ligand of the B cell differentiation antigen CD72.
Ledbetter, J.A. and L.A. Herzenberg, (1979) Immunol. Rev. 47:63.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
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