Mouse CD5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM115
Product Details
Citations (1)
FAQs
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Mouse CD5 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD5 in ELISAs.
Source
Monoclonal Rat IgG2A Clone # 53-7.3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus and spleen cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
8-25 µg/mL
Immunoprecipitation
Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5

CD5, also known as Leu-1, is a glycoprotein expressed on thymocytes, T cells, and the B1a subpopulation of B cells. CD5 is a member of the scavenger receptor cysteine-rich protein superfamily and the ligand of the C-type lectin, CD72. It modulates signaling through the antigen receptors on T and B cells.

Entrez Gene IDs
921 (Human); 12507 (Mouse)
Alternate Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1

Product Datasheets

Citation for Mouse CD5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
    J. Immunol., 2011;186(8):4967-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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