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Mouse CD5 MAb (Clone 53-7.3)

Catalog #: MLDP3 Datasheet / COA / SDS
Newer Version Available: MAB115
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Discontinued Product

MLDP3 has been discontinued and is replaced by MAB115.

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Mouse CD5 MAb (Clone 53-7.3) Summary

Species Reactivity
Mouse
Specificity
This antibody has been shown to react with a mouse monomorphic CD5 (Ly-1), a member of scavenger receptor cysteine-rich protein superfamily.1 CD5 is a membrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells.2 - 6 It is a ligand of the B cell differentiation antigen CD72.
Source
Monoclonal Rat IgG2a Clone # 53-7.3
Label
Unconjugated

Applications

Recommended Concentration
Sample
Cell depletion
This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete CD5+ cells from 1 x 109 bone marrow cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Cell Depletion View Larger

Cell Depletion View Larger

Figure 1:Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems, Catalog # MAB1356; dotted histogram) in bone marrow progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Cell Depletion View Larger

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD5

CD5, also known as Leu-1, is a glycoprotein expressed on thymocytes, mature T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through antigen receptors on T and B cells. CD5 is a member of the scavenger receptor cysteine-rich (SRCR) family.

Entrez Gene IDs
921 (Human); 12507 (Mouse)
Alternate Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1

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