|Detection of CD55/DAF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD55/DAF PE‑conjugated Monoclonal Antibody (Catalog # FAB5376P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.|
CD55, also known as Decay-accelerating Factor/DAF, is a glycoprotein member of the RCA family of molecules. It is found on all blood cells, select epithelium and endothelium, and serves both as a receptor for CD97, and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-70 kDa, 328 amino acid (aa), GPI-linked form (aa 35-362) that is ubiquitously expressed and a 52-56 kDa type I transmembrane (TM) form that shoiws limited expression. The TM form shows the same domain architecture and is 93% aa identical to the GPI-form. At least three GPI gene isoforms exist. One contains a 21 aa 390 substitution for aa 286, a second shows a six aa substitution for aa 339-350, and a third contains a Pro substitution for aa 333-361. Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.
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