Mouse CD55/DAF PE-conjugated Antibody

FAB5376P has been discontinued. View all CD55/DAF products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD55/DAF in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human CD55 or recombinant mouse CD97 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 583905
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD55/DAF
    Met1-Pro359
    Accession # Q61475
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD55/DAF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD55/DAF PE‑conjugated Monoclonal Antibody (Catalog # FAB5376P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD55/DAF

CD55, also known as Decay-accelerating Factor/DAF, is a glycoprotein member of the RCA family of molecules. It is found on all blood cells, select epithelium and endothelium, and serves both as a receptor for CD97, and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-70 kDa, 328 amino acid (aa), GPI-linked form (aa 35-362) that is ubiquitously expressed and a 52-56 kDa type I transmembrane (TM) form that shoiws limited expression. The TM form shows the same domain architecture and is 93% aa identical to the GPI-form. At least three GPI gene isoforms exist. One contains a 21 aa 390 substitution for aa 286, a second shows a six aa substitution for aa 339-350, and a third contains a Pro substitution for aa 333-361. Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.

  • Entrez Gene IDs:
    1604 (Human); 13136 (Mouse)
  • Alternate Names:
    CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD55; CR; CRdecay accelerating factor for complement (CD55, Cromer blood group system); CROMDAFcomplement decay-accelerating factor; DAF; decay accelerating factor for complement; TC
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