Mouse CD8 alpha Alexa Fluor® 405-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects both the alpha and alpha ' chains of mouse CD8 (1).
  • Source
    Monoclonal Rat IgG2A Clone # 53-6.7
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse thymus or spleen
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD8 alpha Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB116V) or (B) Rat IgG2A Alexa Fluor 405 Isotype Control (Catalog # IC006V). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co-receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). The CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3), while CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

  • References:
    1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
    2. Li, Y. et al. (2013) Front. Immunol. 4:206.
    3. Hayakawa, K. et al. (1994) Science 263:1131.
    4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
    5. Rocha, B. et al. (1992) Immunol. Today 13:449.
    6. Wang, J. and J.R. Klein (1994) Science 265:1860.
    7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
    8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
  • Entrez Gene IDs:
    925 (Human); 12525 (Mouse); 24930 (Rat)
  • Alternate Names:
    CD8; CD8A
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 405-conjugated Antibody

Ctrl IC006V
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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