Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell depletion, Inhibition of T Cell Function, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Cell Selection

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 53-6.7
View all CD8 Primary Antibodies »

Product Specifications

Immunogen

Mouse thymus or spleen

Specificity

Detects both the alpha and alpha ' chains of mouse CD8 (1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CD8 alpha Antibody

CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD8 alpha in Mouse Splenocytes.

CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD8 alpha in Mouse Splenocytes.

CD8a was detected in mouse splenocytes using Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD8 alpha by Immunohistochemistry

Detection of CD8 alpha by Immunohistochemistry

T cells and Con A-induced hepatitis. Isotype, CD4 and/or CD8 mAbs (100 µg per mouse) was administrated i.p. to Balb/c mice (n = 8 per group) 24h before Con A injection (20 mg kg–1). The effect of anti-CD4 orCD-8 antibodies administration on depleting these cells in the liver of mice prior injecting Con A was determined by FACS. Representative results were shown in (A). Sera were collected 24 h after Con A injection. Serum ALT (B) and AST (C) levels were measured. The results were presented as the mean ± SD of three separate experiments. ***, p<0.001 vs control. (D) The livers were removed 24 h later. Paraffin sections were stained with hematoxylin and eosin (H&E) staining. Representative liver sections were shown for each group, original magnification: ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23118999), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD8 alpha by Flow Cytometry

Detection of CD8 alpha by Flow Cytometry

Endogenous T cells accumulate in K14E7 skin with an enrichment for CCR6-expressing CD4 T cells.(A) Ear skin tissue was taken from K14E7 mice in addition to control mice expressing the SIY epitope under the K14 promoter. CD4 and CD8 expression was measured on gated CD45+CD3+ cells. Data is representative of at least 4 mice/group (B) The numbers of CD4+ and CD8+ T cells in K14E7 skin or control C57 skin were enumerated per square centimetre of ear skin using flow count beads in flow cytometry. Data represents pooled mice from at least two independent experiments. (C) Gated CD3+ CD4+ T cells from the lymph node or skin of K14E7 mice were analysed for expression of the chemokine receptors, CCR6 and CCR4. The left hand panels show representative plots of isotype and CCR6 antibody staining for CD4+ T cells while the graph summarises chemokine receptor staining representative of 6 mice/group from 3 independent experiments. (D) Both K14E7 mice and C57 mice were analysed for the proliferative marker, Ki67, using intracellular staining of lymphocytes derived from the skin and inguinal lymph nodes (iLN). Naive C57 spleen cells (negative control) or C57 spleen cells treated for 3 days with PMA/Ionomycin (positive control) were also analysed. The K14E7 and C57 data represent 7 mice/group derived from 3 independent experiments while controls are spleen cells from a single mouse in 3 independent experiments. The lower right hand panels are representative plots showing isotype and Ki67 staining in K14E7 or C57 mouse skin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23469070), licensed under a CC0-1.0 license. Not internally tested by R&D Systems.
Detection of CD8 alpha by Flow Cytometry

Detection of CD8 alpha by Flow Cytometry

T cells and Con A-induced hepatitis. Isotype, CD4 and/or CD8 mAbs (100 µg per mouse) was administrated i.p. to Balb/c mice (n = 8 per group) 24h before Con A injection (20 mg kg–1). The effect of anti-CD4 orCD-8 antibodies administration on depleting these cells in the liver of mice prior injecting Con A was determined by FACS. Representative results were shown in (A). Sera were collected 24 h after Con A injection. Serum ALT (B) and AST (C) levels were measured. The results were presented as the mean ± SD of three separate experiments. ***, p<0.001 vs control. (D) The livers were removed 24 h later. Paraffin sections were stained with hematoxylin and eosin (H&E) staining. Representative liver sections were shown for each group, original magnification: ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23118999), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD8 alpha Antibody

Application
Recommended Usage

Cell depletion

Hathcock, K.S. (1991) Current protocols in immunology, pp. 3.4.1. Kruisbeek, A.M. (1991) Current protocols in immunology, pp. 4.1.1.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunoprecipitation

Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.

Inhibition of T Cell Function

This antibody has been used for inhibition of T cell responses to IL-2 (Takahashi, K. et al., 1992, Proc. Natl. Acad. Sci. USA 89:5557 - 5561), MHC class I (Anel, A. et al., 1996, Eur. J. Immunol. 26:2310 - 2319) or antigens (Alexander-Miller, M.A. et al., 1996, J. Exp. Med. 184:485 - 492).

Reviewed Applications

Read 3 reviews rated 4.3 using MAB116 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References

  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

Additional CD8 Products

Product Documents for Mouse CD8 alpha Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD8 alpha Antibody

For research use only

Citations for Mouse CD8 alpha Antibody

Customer Reviews for Mouse CD8 alpha Antibody (3)

4.3 out of 5
3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse CD8 alpha Antibody
    Name: Anonymous
    Application: IHC on Frozen tissue
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 06/05/2018
    Mouse CD8 alpha Antibody MAB116
  • Mouse CD8 alpha Antibody
    Name: Mozhdeh Sojoodi
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Pancreatic cancer tissue
    Species: Mouse
    Verified Customer | Posted 08/08/2017
    1:100 coccentration
    Mouse CD8 alpha Antibody MAB116
  • Mouse CD8 alpha Antibody
    Name: Manuel Flores
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen mouse liver
    Species: Mouse
    Verified Customer | Posted 03/03/2017
    Anti CD8 staining on OCT liver sections from 3 weeks CCl4 treated mouse. Primary Ab CD8 Antibody (53-6.7) MAB116-100 from Novus. Secondary used is anti-rat Cy3. Arrows are pointing at CD8 + cells.
    The staining is clean, and higly specific. Very low background signal.
    Mouse CD8 alpha Antibody MAB116

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Protocols

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FAQs for Mouse CD8 alpha Antibody

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  • Q: Is Mouse CD8 alpha Antibody, Catalog # MAB116, suitable for IHC with paraffin-embedded tissue?

    A: We test this antibody agasint cells and do not have data to show its use against paraffin-embedded tissue. We have citations where customers have used the clone in paraffin-embedded tissue; please see the citation link on the webpage. We are also aware the literature suggests this clone works best with acetone-fixed frozen sections or zinc-fixed paraffin sections.  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4539256/

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