Mouse CD8 alpha Antibody

(2 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects both the alpha and alpha ' chains of mouse CD8 (1).
  • Source
    Monoclonal Rat IgG2A Clone # 53-6.7
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse thymus or spleen
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes
  • Immunoprecipitation
    Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
  • Cell depletion
    Hathcock, K.S. (1991) Current protocols in immunology, pp. 3.4.1. Kruisbeek, A.M. (1991) Current protocols in immunology, pp. 4.1.1.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Inhibition of T Cell Function
    This antibody has been used for inhibition of T cell responses to IL-2 (Takahashi, K. et al., 1992, Proc. Natl. Acad. Sci. USA 89:5557 - 5561), MHC class I (Anel, A. et al., 1996, Eur. J. Immunol. 26:2310 - 2319) or antigens (Alexander-Miller, M.A. et al., 1996, J. Exp. Med. 184:485 - 492).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD8 alpha in Mouse Splenocytes. CD8 alpha was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti‑Rat IgG Secondary Antibody (red; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
CD8 alpha in Mouse Splenocytes. CD8 alpha was detected in mouse splenocytes using Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

  • References:
    1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
    2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
    3. Hayakawa, K. et al. (1994) Science 263:1131.
    4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
    5. Rocha, B. et al. (1992) Immunol. Today 13:449.
    6. Wang, J. and J.R. Klein (1994) Science 265:1860.
    7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
    8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
  • Entrez Gene IDs:
    925 (Human); 12525 (Mouse); 24930 (Rat); 493799 (Feline)
  • Alternate Names:
    CD8; CD8A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
    Authors: Zhang, Hanwei, Chin, Arnold I
    PLoS ONE, 2014;9(4):e94793.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
    Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
    J. Virol., 2007;81(3):1241-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Mouse CD8 alpha  Antibody MAB116
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Very Good
 ICC/IF Mouse Anonymous 08/08/2017
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Immunocytochemistry/Immunofluorescence Mouse CD8 alpha  Antibody MAB116
Immunocytochemistry/Immunofluorescence: Mouse CD8 alpha Antibody [MAB116]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedPancreatic cancer tissue
SpeciesMouse

Other Experimental Details

Other Experimental Details1:100 coccentration

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