|CD8 alpha in Mouse Splenocytes. CD8 alpha was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti‑Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
|CD8 alpha in Mouse Splenocytes. CD8 alpha was detected in mouse splenocytes using Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).
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Immunocytochemistry/Immunofluorescence: Mouse CD8 alpha Antibody [MAB116]
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