Mouse CD8 alpha Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB116T-100UG
Mouse CD8 alpha  Alexa Fluor® 594-conjugated Antibody in Data
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Mouse CD8 alpha Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects both the alpha and alpha ' chains of mouse CD8 (1).
Source
Monoclonal Rat IgG2A Clone # 53-6.7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus or spleen
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD8 alpha  in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD8a Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB116T) or (B) Rat IgG2AAlexa Fluor 594 Isotype Control (Catalog # IC006T). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co-receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). The CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3), while CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References
  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Li, Y. et al. (2013) Front. Immunol. 4:206.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

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