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Mouse CD90/Thy1 Alexa Fluor® 647-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD90/Thy1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD90/Thy1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 778053
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD90/Thy1
    Gln20-Lys130
    Accession # P01831
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry CD90/Thy1 Antibody (778053) [Alexa Fluor® 647]
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Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD90/Thy1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB7335R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD90/Thy1
CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Entrez Gene IDs:
    7070 (Human); 21838 (Mouse)
  • Alternate Names:
    CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 647-conjugated Antibody

 Ctrl IC006R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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