Mouse CD90/Thy1 Antibody Summary
Accession # P01831
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse CD90/Thy1 by Western Blot. Western blot shows lysates of mouse brain tissue and mouse thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD90/Thy1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Citation for Mouse CD90/Thy1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Establishment of inï¿½vitro model of erectile dysfunction for the study of high-glucose-induced angiopathy and neuropathy
Authors: G N Yin
Sample Types: Whole Cells
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