R&D Systems Proteome Profiler™ Mouse Chemokine Array Kit (Catalog # ARY020) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 25 chemokines in a single sample. No specialized equipment is necessary.
Simultaneously detect the levels of these chemokines in a single sample.
Complement Component C5/C5a
CCL3/CCL4 (MIP-1 alpha /MIP-1 beta)
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, and serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of chemokine bound. Chemiluminescence is detected in the same manner as a Western blot.
4 Array Membranes
Detection Antibody Cocktail
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.