Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates and serum.

Key Benefits

  • Detects 25 mouse chemokines simultaneously
  • Requires no specialized equipment
 

Principle of the Array

The Proteome Profiler Mouse Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Cell Stress-Related Proteins?

Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these chemokines in a single sample.
CCL11/Eotaxin CCL6/C10 CXCL11/I-TAC
CCL12/MCP-5 CCL8/MCP-2 CXCL12/SDF-1
CCL2/JE/MCP-1 CCL9/10/MIP-1 gamma CXCL13/BLC/BCA-1
CCL21/6Ckine Chemerin CXCL16
CCL22/MDC Complement Component C5/C5a CXCL2/MIP-2
CCL27/CTACK CX3CL1/Fractalkine CXCL9/MIG
CCL28 CXCL1/KC IL-16
CCL3/CCL4 (MIP-1 alpha /MIP-1 beta) CXCL10/IP-10/CRG-2 LIX
CCL5/RANTES    

 

Assays for analytes represented in the Mouse Chemokine Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CCL21/6Ckine   DY457
CXCL13/BLC/BCA-1 MCX130 DY470
CCL6/C10   DY487
Complement Component C5/C5a   DY2150
CCL28   DY533
Chemerin MCHM00 DY2325
CCL27/CTACK   DY725
CXCL16   DY503
CCL11/Eotaxin MME00 DY420
CX3CL1/Fractalkine MCX310 DY472
IL-16   DY1727
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CCL2/JE/MCP-1 MJE00 DY479
CXCL1/KC MKC00B DY463
CXCL2/MIP-2 MM200 DY452
CCL5/RANTES MMR00 DY478
CXCL12/SDF-1 MCX120 DY460

 

Data Examples
3T3-L1 mouse embryonic fibroblast adipose-like cells
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Figure 1: 3T3-L1 mouse embryonic fibroblast adipose-like cells were untreated or treated with 100 ng/mL LPS for 24 hours.

RAW264.7 mouse monocyte/macrophage cells
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Figure 2: RAW264.7 mouse monocyte/macrophage cells were untreated or treated with 1 µg/mL recombinant mouse IFN-gamma (R&D Systems, Catalog # 485-MI) for 8 hours, followed by 1 µg/mL LPS for 16 hours.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4

  1. Chemotaxis-driven disease-site targeting of therapeutic adult stem cells in dystrophic epidermolysis bullosa
    Stem Cell Res Ther, 2016;7(1):124. 2016
  2. Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice.
    Authors: Evans H, Killoran K, Mitre B, Morris C, Kim S, Mitre E
    J Immunol, 2015;195(7):2973-84. 2015
  3. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36. 2013
  4. Metabolomic profiling reveals mitochondrial-derived lipid biomarkers that drive obesity-associated inflammation.
    Authors: Sampey BP, Freemerman AJ, Zhang J
    PLoS ONE, 2012;7(6):e38812. 2012
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 Proteome Profiler Mouse Chemokine Array Kit ARY020
: Proteome Profiler Mouse Chemokine Array Kit [ARY020]

Other Experimental Details

Other Experimental DetailsTotal lysate of mouse neutrophil (published data. Science. 2015 Sep 4;349(6252):aaa4352)

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