Proteome Profiler Mouse Chemokine Array Kit

Contains 4 membranes - each spotted in duplicate with 25 different chemokine antibodies
Catalog # Availability Size / Price Qty
Detection of Multiple Analytes in Cell Culture Supernate with the Mouse Chemokine Array.
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Product Details
Citations (25)
Supplemental Products
Reviews (6)

Proteome Profiler Mouse Chemokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates and serum.


Troubleshooting Guide

Key Benefits

  • Detects 25 mouse chemokines simultaneously
  • Requires no specialized equipment

Principle of the Array

The Proteome Profiler Mouse Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Cell Stress-Related Proteins?

Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.


Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.


Simultaneously detect the levels of these chemokines in a single sample.
CCL11/Eotaxin CCL6/C10 CXCL11/I-TAC
CCL2/JE/MCP-1 CCL9/10/MIP-1 gamma CXCL13/BLC/BCA-1
CCL21/6Ckine Chemerin CXCL16
CCL22/MDC Complement Component C5/C5a CXCL2/MIP-2
CCL27/CTACK CX3CL1/Fractalkine CXCL9/MIG
CCL3/CCL4 (MIP-1 alpha /MIP-1 beta) CXCL10/IP-10/CRG-2 LIX


Assays for analytes represented in the Mouse Chemokine Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CCL21/6Ckine   DY457
CCL6/C10   DY487
Complement Component C5/C5a   DY2150
CCL28   DY533
Chemerin MCHM00 DY2325
CXCL16   DY503
CCL11/Eotaxin MME00 DY420
CX3CL1/Fractalkine MCX310 DY472
IL-16   DY1727
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CXCL2/MIP-2 MM200 DY452
CXCL12/SDF-1 MCX120 DY460


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Scientific Data

Detection of Multiple Analytes in Cell Culture Supernate with the Mouse Chemokine Array. 3T3-L1 mouse embryonic fibroblast adipose-like cells were untreated or treated with 100 ng/mL LPS for 24 hours.

Detection of Multiple Analytes in Cell Culture Supernate with the Mouse Chemokine Array. RAW264.7 mouse monocyte/macrophage cells were untreated or treated with 1 µg/mL recombinant mouse IFN-gamma (R&D Systems, Catalog # 485-MI) for 8 hours, followed by 1 µg/mL LPS for 16 hours.

Citations for Proteome Profiler Mouse Chemokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death
    Authors: M Scur, AB Mahmoud, S Dey, F Abdalbarri, I Stylianide, D Medina-Lun, GS Gamage, A Woblistin, ANM Wilson, HS Zein, A Stueck, A Wight, OA Aguilar, F Di Cara, BD Parsons, MMA Rahim, JR Carlyle, AP Makrigiann
    Nature Communications, 2022;13(1):7272.  2022
  2. Chemokine CCL9 Is Upregulated Early in Chronic Kidney Disease and Counteracts Kidney Inflammation and Fibrosis
    Authors: C Hemmers, C Schulte, J Wollenhaup, DWL Wong, E Harlacher, S Orth-Alamp, BM Klinkhamme, SH Schirmer, M Böhm, N Marx, T Speer, P Boor, J Jankowski, H Noels
    Biomedicines, 2022;10(2):.  2022
  3. Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance
    Authors: Y Deng, X Xia, Y Zhao, Z Zhao, C Martinez, W Yin, J Yao, Q Hang, W Wu, J Zhang, Y Yu, W Xia, F Yao, D Zhao, Y Sun, H Ying, MC Hung, L Ma
    Nature Communications, 2021;12(1):7041.  2021
  4. High-Fat Diet-Induced Obese Effects of Adipocyte-Specific CXCR2 Conditional Knockout in the Peritoneal Tumor Microenvironment of Ovarian Cancer
    Authors: D Choe, ES Lee, A Beeghly-Fa, AJ Wilson, MM Whalen, SE Adunyah, DS Son
    Cancers, 2021;13(19):.  2021
  5. Discovery of chemerin as the new chemoattractant of human mesenchymal stem cells
    Authors: I Kim, H Park, I Hwang, D Moon, H Yun, EJ Lee, HS Kim
    Cell & bioscience, 2021;11(1):120.  2021
  6. In situ neutrophil efferocytosis shapes T cell immunity to influenza infection
    Authors: K Lim, TH Kim, A Trzeciak, AM Amitrano, EC Reilly, H Prizant, DJ Fowell, DJ Topham, M Kim
    Nat. Immunol., 2020;0(0):.  2020
  7. In situ neutrophil efferocytosis shapes T cell immunity to influenza infection
    Authors: K Lim, TH Kim, A Trzeciak, AM Amitrano, EC Reilly, H Prizant, DJ Fowell, DJ Topham, M Kim
    Nat. Immunol., 2020;0(0):.  2020
  8. CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury
    Authors: J Giri, R Das, E Nylen, R Chinnadura, J Galipeau
    Cell Rep, 2020;30(6):1923-1934.e4.  2020
  9. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma
    Authors: GM Wiedemann, C Aithal, A Kraechan, C Heise, BL Cadilha, J Zhang, P Duewell, R Ballotti, S Endres, C Bertolotto, S Kobold
    Transl Oncol, 2018;12(2):350-360.  2018
  10. Chlamydia and Lipids Engage a Common�Signaling Pathway That Promotes Atherogenesis
    Authors: S Chen, K Shimada, TR Crother, E Erbay, PK Shah, M Arditi
    J. Am. Coll. Cardiol., 2018;71(14):1553-1570.  2018
  11. RNA-based micelles: A novel platform for chemotherapeutic drug loading and delivery
    Authors: Y Shu, H Yin, M Rajabi, H Li, M Vieweger, S Guo, D Shu, P Guo
    J Control Release, 2018;0(0):.  2018
  12. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018;172(5):1022-1037.e14.  2018
  13. Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury
    Authors: JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond
    J Neuroinflammation, 2018;15(1):192.  2018
  14. The hyaluronic acid-rich node and duct system is a structure organized for innate immunity and mediates the local inflammation
    Authors: BK Choi, SH Hwang, YI Kim, R Singh, BS Kwon
    Cytokine, 2018;0(0):.  2018
  15. Myeloid p38? signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis
    Authors: C Youssif, M Cubillos-R, M Comalada, E Llonch, C Perna, N Djouder, AR Nebreda
    EMBO Mol Med, 2018;0(0):.  2018
  16. Stromal Cell-Derived Factor 1 Mediates Immune Cell Attraction upon Urinary Tract Infection
    Authors: B Isaacson, T Hadad, A Glasner, C Gur, Z Granot, G Bachrach, O Mandelboim
    Cell Rep, 2017;20(1):40-47.  2017
  17. Sitagliptin, a dipeptidyl peptidase-4 inhibitor, suppresses CXCL5 and SDF-1 and does not accelerate intestinal neoplasia formation in Apc(Min/+) mice fed a high-fat diet
    Authors: K Fujiwara, T Inoue, Y Henmi, Y Hirata, Y Naka, A Hara, K Kakimoto, S Nouda, T Okada, K Kawakami, T Takeuchi, K Higuchi
    Oncol Lett, 2017;14(4):4355-4360.  2017
  18. SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts
    Authors: M Muramatsu, L Gao, J Peresie, B Balderman, S Akakura, IH Gelman
    Oncotarget, 2017;8(41):70281-70298.  2017
  19. Effect of low-level CO2 on innate inflammatory protein response to organic dust from swine confinement barns
    Authors: D Schneberge, JM DeVasure, KL Bailey, DJ Romberger, TA Wyatt
    J Occup Med Toxicol, 2017;12(0):9.  2017
  20. Unliganded estrogen receptor alpha regulates vascular cell function and gene expression
    Authors: Richard H Karas
    Mol. Cell. Endocrinol., 2016;442(0):12-23.  2016
  21. Novel chemokine-like activities of histones in tumor metastasis
    Authors: R Chen, Y Xie, X Zhong, Y Fu, Y Huang, Y Zhen, P Pan, H Wang, DL Bartlett, TR Billiar, MT Lotze, HJ Zeh, XG Fan, D Tang, R Kang
    Oncotarget, 2016;7(38):61728-61740.  2016
  22. Chemotaxis-driven disease-site targeting of therapeutic adult stem cells in dystrophic epidermolysis bullosa
    Stem Cell Res Ther, 2016;7(1):124.  2016
  23. Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice.
    Authors: Evans H, Killoran K, Mitre B, Morris C, Kim S, Mitre E
    J Immunol, 2015;195(7):2973-84.  2015
  24. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.  2013
  25. Metabolomic profiling reveals mitochondrial-derived lipid biomarkers that drive obesity-associated inflammation.
    Authors: Sampey BP, Freemerman AJ, Zhang J
    PLoS ONE, 2012;7(6):e38812.  2012


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Total lysate of mouse neutrophil (published data. Science. 2015 Sep 4;349(6252):aaa4352)