The Proteome Profiler Mouse Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the levels of these chemokines in a single sample.|
|CCL22/MDC||Complement Component C5/C5a||CXCL2/MIP-2|
|CCL3/CCL4 (MIP-1 alpha /MIP-1 beta)||CXCL10/IP-10/CRG-2||LIX|
|Analyte||Quantikine® ELISA Kits||DuoSet® ELISA Development Systems|
|Complement Component C5/C5a||DY2150|
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Figure 1: 3T3-L1 mouse embryonic fibroblast adipose-like cells were untreated or treated with 100 ng/mL LPS for 24 hours.
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Figure 2: RAW264.7 mouse monocyte/macrophage cells were untreated or treated with 1 µg/mL recombinant mouse IFN-gamma (R&D Systems, Catalog # 485-MI) for 8 hours, followed by 1 µg/mL LPS for 16 hours.
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: Proteome Profiler Mouse Chemokine Array Kit [ARY020]
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