Proteome Profiler Mouse Chemokine Array Kit

Contains 4 membranes - each spotted in duplicate with 25 different chemokine antibodies
  (12 citations)
(3 Reviews)
Datasheet / CoA / SDS
Product Details
Citations (12)
Supplemental Products
Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates and serum.


Troubleshooting Guide

Key Benefits

  • Detects 25 mouse chemokines simultaneously
  • Requires no specialized equipment

Principle of the Array

The Proteome Profiler Mouse Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Cell Stress-Related Proteins?

Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.


Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.


Simultaneously detect the levels of these chemokines in a single sample.
CCL11/Eotaxin CCL6/C10 CXCL11/I-TAC
CCL2/JE/MCP-1 CCL9/10/MIP-1 gamma CXCL13/BLC/BCA-1
CCL21/6Ckine Chemerin CXCL16
CCL22/MDC Complement Component C5/C5a CXCL2/MIP-2
CCL27/CTACK CX3CL1/Fractalkine CXCL9/MIG
CCL3/CCL4 (MIP-1 alpha /MIP-1 beta) CXCL10/IP-10/CRG-2 LIX


Assays for analytes represented in the Mouse Chemokine Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CCL21/6Ckine   DY457
CCL6/C10   DY487
Complement Component C5/C5a   DY2150
CCL28   DY533
Chemerin MCHM00 DY2325
CXCL16   DY503
CCL11/Eotaxin MME00 DY420
CX3CL1/Fractalkine MCX310 DY472
IL-16   DY1727
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CXCL2/MIP-2 MM200 DY452
CXCL12/SDF-1 MCX120 DY460


Data Examples
3T3-L1 mouse embryonic fibroblast adipose-like cells
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Figure 1: 3T3-L1 mouse embryonic fibroblast adipose-like cells were untreated or treated with 100 ng/mL LPS for 24 hours.

RAW264.7 mouse monocyte/macrophage cells
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Figure 2: RAW264.7 mouse monocyte/macrophage cells were untreated or treated with 1 µg/mL recombinant mouse IFN-gamma (R&D Systems, Catalog # 485-MI) for 8 hours, followed by 1 µg/mL LPS for 16 hours.

Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Sample Type
  1. The hyaluronic acid-rich node and duct system is a structure organized for innate immunity and mediates the local inflammation
    Authors: BK Choi, SH Hwang, YI Kim, R Singh, BS Kwon
    Cytokine, 2018;0(0):.  2018
  2. Myeloid p38? signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis
    Authors: C Youssif, M Cubillos-R, M Comalada, E Llonch, C Perna, N Djouder, AR Nebreda
    EMBO Mol Med, 2018;0(0):.  2018
  3. Sitagliptin, a dipeptidyl peptidase-4 inhibitor, suppresses CXCL5 and SDF-1 and does not accelerate intestinal neoplasia formation in Apc(Min/+) mice fed a high-fat diet
    Authors: K Fujiwara, T Inoue, Y Henmi, Y Hirata, Y Naka, A Hara, K Kakimoto, S Nouda, T Okada, K Kawakami, T Takeuchi, K Higuchi
    Oncol Lett, 2017;14(4):4355-4360.  2017
  4. Stromal Cell-Derived Factor 1 Mediates Immune Cell Attraction upon Urinary Tract Infection
    Authors: B Isaacson, T Hadad, A Glasner, C Gur, Z Granot, G Bachrach, O Mandelboim
    Cell Rep, 2017;20(1):40-47.  2017
  5. SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts
    Authors: M Muramatsu, L Gao, J Peresie, B Balderman, S Akakura, IH Gelman
    Oncotarget, 2017;8(41):70281-70298.  2017
  6. Effect of low-level CO2 on innate inflammatory protein response to organic dust from swine confinement barns
    Authors: D Schneberge, JM DeVasure, KL Bailey, DJ Romberger, TA Wyatt
    J Occup Med Toxicol, 2017;12(0):9.  2017
  7. Chemotaxis-driven disease-site targeting of therapeutic adult stem cells in dystrophic epidermolysis bullosa
    Stem Cell Res Ther, 2016;7(1):124.  2016
  8. Novel chemokine-like activities of histones in tumor metastasis
    Authors: R Chen, Y Xie, X Zhong, Y Fu, Y Huang, Y Zhen, P Pan, H Wang, DL Bartlett, TR Billiar, MT Lotze, HJ Zeh, XG Fan, D Tang, R Kang
    Oncotarget, 2016;7(38):61728-61740.  2016
  9. Unliganded estrogen receptor alpha regulates vascular cell function and gene expression
    Authors: Richard H Karas
    Mol. Cell. Endocrinol., 2016;442(0):12-23.  2016
  10. Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice.
    Authors: Evans H, Killoran K, Mitre B, Morris C, Kim S, Mitre E
    J Immunol, 2015;195(7):2973-84.  2015
  11. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.  2013
  12. Metabolomic profiling reveals mitochondrial-derived lipid biomarkers that drive obesity-associated inflammation.
    Authors: Sampey BP, Freemerman AJ, Zhang J
    PLoS ONE, 2012;7(6):e38812.  2012
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Average Rating: 5 (Based on 3 reviews)

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Images Ratings Applications Species Reviewed By Date Details
  Anonymous 07/18/2018
  Anonymous 10/17/2017

Other Experimental Details

Other Experimental Details Mouse peritoneal macrophages
 Proteome Profiler Mouse Chemokine Array Kit ARY020 Array ARY020
  Anonymous 09/29/2016
Image Details
 Proteome Profiler Mouse Chemokine Array Kit ARY020
: Proteome Profiler Mouse Chemokine Array Kit [ARY020].

Other Experimental Details

Other Experimental Details Total lysate of mouse neutrophil (published data. Science. 2015 Sep 4;349(6252):aaa4352)

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