A membrane-based antibody array for the parallel determination of the relative levels of selected mouse chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates and serum.
The Proteome Profiler Mouse Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Cell Stress-Related Proteins?
Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
4 Array Membranes
Detection Antibody Cocktail
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these chemokines in a single sample.
Complement Component C5/C5a
CCL3/CCL4 (MIP-1 alpha /MIP-1 beta)
Assays for analytes represented in the Mouse Chemokine Array Kit
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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