Proteome Profiler Mouse Chemokine Array Kit
Proteome Profiler Mouse Chemokine Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected mouse chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates and serum.
- Detects 25 mouse chemokines simultaneously
- Requires no specialized equipment
Principle of the Array
The Proteome Profiler Mouse Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Cell Stress-Related Proteins?
Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the levels of these chemokines in a single sample.|
|CCL22/MDC||Complement Component C5/C5a||CXCL2/MIP-2|
|CCL3/CCL4 (MIP-1 alpha /MIP-1 beta)||CXCL10/IP-10/CRG-2||LIX|
Assays for analytes represented in the Mouse Chemokine Array Kit
|Analyte||Quantikine® ELISA Kits||DuoSet® ELISA Development Systems|
|Complement Component C5/C5a||DY2150|
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Figure 1: 3T3-L1 mouse embryonic fibroblast adipose-like cells were untreated or treated with 100 ng/mL LPS for 24 hours.
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Figure 2: RAW264.7 mouse monocyte/macrophage cells were untreated or treated with 1 µg/mL recombinant mouse IFN-gamma (R&D Systems, Catalog # 485-MI) for 8 hours, followed by 1 µg/mL LPS for 16 hours.
Preparation and Storage
Citations for Proteome Profiler Mouse Chemokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Myeloid p38? signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis
Authors: C Youssif, M Cubillos-R, M Comalada, E Llonch, C Perna, N Djouder, AR Nebreda
EMBO Mol Med, 2018;0(0):. 2018
The hyaluronic acid-rich node and duct system is a structure organized for innate immunity and mediates the local inflammation
Authors: BK Choi, SH Hwang, YI Kim, R Singh, BS Kwon
Cytokine, 2018;0(0):. 2018
Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury
Authors: JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond
J Neuroinflammation, 2018;15(1):192. 2018
Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma
Authors: GM Wiedemann, C Aithal, A Kraechan, C Heise, BL Cadilha, J Zhang, P Duewell, R Ballotti, S Endres, C Bertolotto, S Kobold
Transl Oncol, 2018;12(2):350-360. 2018
Effect of low-level CO2 on innate inflammatory protein response to organic dust from swine confinement barns
Authors: D Schneberge, JM DeVasure, KL Bailey, DJ Romberger, TA Wyatt
J Occup Med Toxicol, 2017;12(0):9. 2017
SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts
Authors: M Muramatsu, L Gao, J Peresie, B Balderman, S Akakura, IH Gelman
Oncotarget, 2017;8(41):70281-70298. 2017
Sitagliptin, a dipeptidyl peptidase-4 inhibitor, suppresses CXCL5 and SDF-1 and does not accelerate intestinal neoplasia formation in Apc(Min/+) mice fed a high-fat diet
Authors: K Fujiwara, T Inoue, Y Henmi, Y Hirata, Y Naka, A Hara, K Kakimoto, S Nouda, T Okada, K Kawakami, T Takeuchi, K Higuchi
Oncol Lett, 2017;14(4):4355-4360. 2017
Stromal Cell-Derived Factor 1 Mediates Immune Cell Attraction upon Urinary Tract Infection
Authors: B Isaacson, T Hadad, A Glasner, C Gur, Z Granot, G Bachrach, O Mandelboim
Cell Rep, 2017;20(1):40-47. 2017
Chemotaxis-driven disease-site targeting of therapeutic adult stem cells in dystrophic epidermolysis bullosa
Stem Cell Res Ther, 2016;7(1):124. 2016
Novel chemokine-like activities of histones in tumor metastasis
Authors: R Chen, Y Xie, X Zhong, Y Fu, Y Huang, Y Zhen, P Pan, H Wang, DL Bartlett, TR Billiar, MT Lotze, HJ Zeh, XG Fan, D Tang, R Kang
Oncotarget, 2016;7(38):61728-61740. 2016
Unliganded estrogen receptor alpha regulates vascular cell function and gene expression
Authors: Richard H Karas
Mol. Cell. Endocrinol., 2016;442(0):12-23. 2016
Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice.
Authors: Evans H, Killoran K, Mitre B, Morris C, Kim S, Mitre E
J Immunol, 2015;195(7):2973-84. 2015
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
J Immunol, 2013;190(10):5329-36. 2013
Metabolomic profiling reveals mitochondrial-derived lipid biomarkers that drive obesity-associated inflammation.
Authors: Sampey BP, Freemerman AJ, Zhang J
PLoS ONE, 2012;7(6):e38812. 2012
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Mouse peritoneal macrophages