Mouse Chitinase 3-like 1 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Chitinase 3-like 1 (CHI3L1) in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human CHI3L1.
  • Source
    Monoclonal Rat IgG2B Clone # 321924
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Chitinase 3-like 1
    Tyr22-Ala381
    Accession # Q61362
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Chitinase 3‑like 1 by Western Blot. Western blot shows lysates of mouse lung tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Chitinase 3‑like 1 Monoclonal Antibody (Catalog # MAB2649) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Chitinase 3‑like 1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chitinase 3-like 1

Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).

  • References:
    1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
    2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
    3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
    4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
    5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
    6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
    7. Shao, R. et al. (2009) Oncogene 28:4456.
    8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
    9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
    10. Letuve, S. et al. (2008) J Immunol. 181:5167.
    11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
    12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
    13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
    14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
    15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
    16. Lee, C.G. et al. (2009) J. Exp. Med. 206:1149.
  • Entrez Gene IDs:
    1116 (Human); 12654 (Mouse)
  • Alternate Names:
    AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Stat3 downstream gene product chitinase 3-like 1 is a potential biomarker of inflammation-induced lung cancer in multiple mouse lung tumor models and humans.
    Authors: Yan, Cong, Ding, Xinchun, Wu, Lingyan, Yu, Menggang, Qu, Peng, Du, Hong
    PLoS ONE, 2013;8(4):e61984.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  2. Breast regression protein-39 (BRP-39) promotes dendritic cell maturation in vitro and enhances Th2 inflammation in murine model of asthma.
    Authors: Xu Q, Chai S, Qian Y, Zhang M, Wang K
    Acta Pharmacol Sin, 2012;33(12):1525-32.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
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Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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