Mouse Chitotriosidase/CHIT1 Antibody

Catalog #: AF5325
1 Citation Datasheet / COA / SDS

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AF5325
AF5325-SP

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Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Chitotriosidase/CHIT1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Chitotriosidase/CHIT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human CHIT1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Chitotriosidase/CHIT1
Ala22-Ser464
Accession # Q9D7Q1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Chitotriosidase/CHIT1 (Catalog # 5325-GH)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Chitotriosidase/CHIT1 (Catalog # 5325-GH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitotriosidase/CHIT1

Chitotriosidase, encoded by the mouse CHIT1 gene, is a typical member of the chitinase family (1). It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract (2). CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form (2‑4). Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease (5). The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme (ACE) (5). Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (6).

References

Zheng, T. et al. (2005) Gene 357:37.
Boot, R.G. et al. (2006) J. Histochem. Cytochem. 53:1283.
Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Entrez Gene IDs

1118 (Human)

Alternate Names

CHI3; CHIT1; CHITCHI3; chitinase 1 (chitotriosidase); chitinase-1; Chitotriosidase; chitotriosidase-1; EC 3.2.1.14; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Chitotriosidase/CHIT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease
Authors: SJ Van Dyken, HE Liang, RP Naikawadi, PG Woodruff, PJ Wolters, DJ Erle, RM Locksley
Cell, 2017-04-20;169(3):497-509.e13.