Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Chitotriosidase/CHIT1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Chitotriosidase/CHIT1
Ala22-Ser464
Accession # Q9D7Q1

Specificity

Detects mouse Chitotriosidase/CHIT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human CHIT1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Chitotriosidase/CHIT1 Antibody

Detection of Mouse Chitotriosidase/CHIT1 by Western Blot

Detection of Mouse Chitotriosidase/CHIT1 by Western Blot

OATD-01 ameliorates TGF beta 1, IL-13, and CHIT1 in chronic HDM model in a dose-dependent fashion. (A) mRNA expression of Il13 in the lungs and (B) active TGF beta 1 levels in BAL fluid in mice with chronic HDM (7 week) treated with OATD-01 (3 and 30 mg/kg; PO; single dose) or vehicle control. (C) Number of macrophages in BAL fluid in mice with chronic HDM (7-week-long) treated with OATD-01 (3 and 30 mg/kg; PO; single dose) or vehicle control. The level of CHIT1 protein in BAL fluid of control and HDM-instilled mice treated with vehicle or OATD-01 (30 mg/kg) in chronic airway inflammation model as evaluated by (D) Western blot and (E) corresponding densitometry analysis. Data presented as mean ± s.e.m. p-values < 0.05 were considered as statistically significant and presented as * for p < 0.05, ** for p < 0.01, *** for p < 0.001, and **** for p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36902148), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Chitotriosidase/CHIT1 by Western Blot

Detection of Mouse Chitotriosidase/CHIT1 by Western Blot

OATD-01 ameliorates TGF beta 1, IL-13, and CHIT1 in chronic HDM model in a dose-dependent fashion. (A) mRNA expression of Il13 in the lungs and (B) active TGF beta 1 levels in BAL fluid in mice with chronic HDM (7 week) treated with OATD-01 (3 and 30 mg/kg; PO; single dose) or vehicle control. (C) Number of macrophages in BAL fluid in mice with chronic HDM (7-week-long) treated with OATD-01 (3 and 30 mg/kg; PO; single dose) or vehicle control. The level of CHIT1 protein in BAL fluid of control and HDM-instilled mice treated with vehicle or OATD-01 (30 mg/kg) in chronic airway inflammation model as evaluated by (D) Western blot and (E) corresponding densitometry analysis. Data presented as mean ± s.e.m. p-values < 0.05 were considered as statistically significant and presented as * for p < 0.05, ** for p < 0.01, *** for p < 0.001, and **** for p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36902148), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Chitotriosidase/CHIT1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Chitotriosidase/CHIT1 (Catalog # 5325-GH), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Chitotriosidase/CHIT1 (Catalog # 5325-GH)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chitotriosidase/CHIT1

Chitotriosidase, encoded by the mouse CHIT1 gene, is a typical member of the chitinase family (1). It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract (2). CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form (2‑4). Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease (5). The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme (ACE) (5). Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (6).

References

  1. Zheng, T. et al. (2005) Gene 357:37.
  2. Boot, R.G. et al. (2006) J. Histochem. Cytochem. 53:1283.
  3. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
  4. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
  5. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
  6. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Alternate Names

CHI3, CHIT1

Entrez Gene IDs

1118 (Human)

Gene Symbol

CHIT1

UniProt

Q9D7Q1

Additional Chitotriosidase/CHIT1 Products

Product Documents for Mouse Chitotriosidase/CHIT1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Chitotriosidase/CHIT1 Antibody

For research use only

Citations for Mouse Chitotriosidase/CHIT1 Antibody

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