Mouse Chitotriosidase/CHIT1 Antibody

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Product Details
Citations (1)
Supplemental Products

Mouse Chitotriosidase/CHIT1 Antibody Summary

Species Reactivity
Detects mouse Chitotriosidase/CHIT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human CHIT1 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Chitotriosidase/CHIT1
Accession # Q9D7Q1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Chitotriosidase/CHIT1 (Catalog # 5325-GH)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Chitotriosidase/CHIT1 (Catalog # 5325-GH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitotriosidase/CHIT1

Chitotriosidase, encoded by the mouse CHIT1 gene, is a typical member of the chitinase family (1). It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract (2). CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form (2‑4). Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease (5). The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme (ACE) (5). Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (6).

  1. Zheng, T. et al. (2005) Gene 357:37.
  2. Boot, R.G. et al. (2006) J. Histochem. Cytochem. 53:1283.
  3. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
  4. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
  5. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
  6. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.
Entrez Gene IDs
1118 (Human)
Alternate Names
CHI3; CHIT1; CHITCHI3; chitinase 1 (chitotriosidase); chitinase-1; Chitotriosidase; chitotriosidase-1; EC; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

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Citation for Mouse Chitotriosidase/CHIT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease
    Authors: SJ Van Dyken, HE Liang, RP Naikawadi, PG Woodruff, PJ Wolters, DJ Erle, RM Locksley
    Cell, 2017-04-20;169(3):497-509.e13.


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