Mouse CL-P1/COLEC12 APC-conjugated Antibody

FAB3130A has been discontinued. View all CL-P1/COLEC12 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CL‑P1/COLEC12 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CL‑P1/COLEC12
    Ala101-Leu742
    Accession # Q8K4Q8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CL‑P1/COLEC12 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CL‑P1/COLEC12 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3130A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CL-P1/COLEC12

Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Collectin Placenta 1 (CL-P1), also known as Collectin sub-family member 12 and Scavenger Receptor with C-type Lectin type I (SRCL), is a 140 kDa member of the collectin family of glycoproteins. With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL‑L1 (Collectin Liver-1) is the only known cytoplasmic collectin (1). Mouse CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that includes an N-terminal 39 aa cytoplasmic domain, an 18 aa transmembrane segment, and a 685 aa C-terminal extracellular domain. The short cytoplasmic domain contains an internalization motif (Y-K-R-F), while the ECD is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure, and a C‑type lectin/Carbohydrate Recognition Domain (CRD) (5, 6). Unlike human CL-P1, no splice variants of mouse CL-P1 have been described (5, 7). Trimerization of CL‑P1 is mediated by its collagen-like and coiled-coil helical domains (1, 6). Within the ECD, mouse CL-P1 shares 88%, 89%, 92%, and 98% aa sequence identity with bovine, canine, human, and rat CL-P1, respectively. The CRD shares 23‑27% aa sequence identity with the CRD of collectins CL-L1, collectin sub-family member 11, MBL, SP-A1, and SP-D. Notably, this CRD recognizes galactose and fucose within the context of asialo-orosomucoids associated with the Lewisx epitope (8, 9). CL‑P1 is expressed in vascular endothelial cells and may play a role in bacterial recognition or as a scavenger receptor for desialylated glycoproteins (6, 8).

  • References:
    1. van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
    2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
    3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
    4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
    5. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
    6. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
    7. Nakamura, K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
    8. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
    9. Yoshida, T. et al. (2003) J. Biochem. 133:271.
  • Long Name:
    Collectin Placenta 1
  • Entrez Gene IDs:
    81035 (Human); 140792 (Mouse); 361289 (Rat)
  • Alternate Names:
    CL-P1; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); COLEC12; EC 2.7.1.78; hClp1; HEABATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; NSR2; Polynucleotide kinase Clp1; polyribonucleotide 5'-hydroxyl-kinase Clp1; Pre-mRNA cleavage complex II protein Clp1; SCARA4; SRCL Type I
Related Research Areas

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