Mouse Collagen II Antibody [Biotin]

R&D Systems | Catalog # BAF3615

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Collagen II Primary Antibodies »

Product Specifications

Immunogen

Mouse sternum-derived Collagen II

Specificity

Detects mouse Collagen II in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse Collagen II Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Mouse Collagen II

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collagen II

Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Entrez Gene IDs

1280 (Human); 12824 (Mouse); 25412 (Rat)

Gene Symbol

COL2A1

Additional Collagen II Products

Product Documents for Mouse Collagen II Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Mouse Collagen II Antibody [Biotin]

For research use only

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