Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.
Mouse Collagen II Biotinylated Antibody
R&D Systems | Catalog # BAF3615
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Mouse sternum-derived Collagen II
Specificity
Detects mouse Collagen II in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse Collagen II Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Mouse Collagen II
Sample: Mouse Collagen II
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Collagen II
Alternate Names
Chondrocalcin, COL2A1, SEDC
Gene Symbol
COL2A1
Additional Collagen II Products
Product Documents for Mouse Collagen II Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Collagen II Biotinylated Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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