Mouse Complement Component C5a Antibody Summary
Accession # P06684
Complete Your Research
Complete Your Experiment
Mouse Complement Component C5a Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody. Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl- beta -D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl- beta -D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Complement Component C5a Monoclonal Antibody (Catalog # MAB21501). The ND50 is typically 0.1-0.5 µg/mL in the presence of cytochalasin-B.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5a
Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).
- Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
- DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
- Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
- Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
- Okinaga, S. et al. (2003) Biochemistry 42:9406.
Citations for Mouse Complement Component C5a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells
Authors: M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu
Journal of Neuroinflammation, 2022;19(1):182.
Sample Types: In Vivo
Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara.
Authors: Price P, Banki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann M
J Immunol, 2015;194(3):1164-8.
Sample Types: BALF
Applications: Western Blot
Real-Time Imaging of Interactions of Neutrophils with Cryptococcus neoformans Demonstrates a Crucial Role of Complement C5a-C5aR Signaling.
Authors: Sun D, Zhang M, Liu G, Wu H, Zhu X, Zhou H, Shi M
Infect Immun, 2015;84(1):216-29.
Sample Types: Whole Cells
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse Complement Component C5a Antibody
Average Rating: 3 (Based on 2 Reviews)
Have you used Mouse Complement Component C5a Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Diffuse staining also present in C5 KO.
OCT embedded frozen tissue, acetone fixed.
1/500 primary concentration with a 1/400 goat anti-rat IgG AlexaFluor555 secondary.