Mouse Complement Component C5a Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Complement Component C5a in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse Complement Component C3d, recombinant human (rh) Complement Component C3a, or rhComplement Component C5a.
  • Source
    Monoclonal Rat IgG2A Clone # 295108
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Complement Component C5a
    Asn679-Arg755
    Accession # P06684
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse Complement Component C5a (Catalog # 2150-C5)
    • Mouse Complement Component C5a Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse Complement Component C5a Antibody (Catalog # MAB21501)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Complement Component C5a Biotinylated Antibody (Catalog # BAF2150)
  • ELISA Standard
     
    Recombinant Mouse Complement Component C5a Protein (Catalog # 2150-C5)
  • Neutralization
    Measured by its ability to neutralize Complement Component C5a-induced N-acetyl-beta -D-glucosaminidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line. Klos, A. et al. (1992) Biochemistry 31:11274. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse Complement Component C5a and cytochalasin‑B.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody. Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces
N‑acetyl- beta -D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N‑Acetyl- beta -D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Complement Component C5a Monoclonal Antibody (Catalog # MAB21501). The ND50 is typically 0.1-0.5 µg/mL in the presence of cytochalasin‑B.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5a

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

  • References:
    1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
    2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
    3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
    4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
    5. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  • Entrez Gene IDs:
    727 (Human); 15139 (Mouse)
  • Alternate Names:
    C5; C5A; Complement Component C5a; HE
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara.
    Authors: Price P, Banki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann M
    J Immunol, 2015;194(3):1164-8.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  2. Real-Time Imaging of Interactions of Neutrophils with Cryptococcus neoformans Demonstrates a Crucial Role of Complement C5a-C5aR Signaling.
    Authors: Sun D, Zhang M, Liu G, Wu H, Zhu X, Zhou H, Shi M
    Infect Immun, 2015;84(1):216-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Rat IgG2A Isotype Control

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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