Mouse Complement Factor D/Adipsin Antibody Summary
Accession # P03953
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse adipose tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor D/Adipsin Monoclonal Antibody (Catalog # MAB5430) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Complement Factor D/Adipsin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor D/Adipsin
Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Complement Factor D contains one peptidase S1 domain that cleaves an Arg‑Lys bond. There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse Complement Factor D shares 85% and 68% aa identity with rat and human Complement Factor D, respectively.
Citations for Mouse Complement Factor D/Adipsin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Toll-like Receptor 2 Facilitates Oxidative Damage-Induced Retinal Degeneration
Authors: K Mulfaul, E Ozaki, N Fernando, K Brennan, KR Chirco, E Connolly, C Greene, A Maminishki, RG Salomon, M Linetsky, R Natoli, RF Mullins, M Campbell, SL Doyle
Cell Rep, 2020;30(7):2209-2224.e5.
Sample Types: In Vivo
Fat-Produced Adipsin Regulates Inflammatory Arthritis
Authors: Y Li, W Zou, JR Brestoff, N Rohatgi, X Wu, JP Atkinson, CA Harris, SL Teitelbaum
Cell Rep, 2019;27(10):2809-2816.e3.
Species: Transgenic Mouse
Sample Types: Serum
Applications: Western Blot
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