Mouse Complement Factor D/Adipsin Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Complement Factor D/Adipsin by Western Blot.
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Product Details
Citations (2)
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Mouse Complement Factor D/Adipsin Antibody Summary

Species Reactivity
Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Complement Factors B, D,H, I or rhGranzyme A is observed.
Monoclonal Rat IgG1 Clone # 577620
Protein A or G purified from hybridoma culture supernatant
NS0 cell-derived recombinant mouse Complement Factor D/Adipsin
Accession # P03953
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse Complement Factor D, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Complement Factor D/Adipsin antibody by Western Blot. View Larger

Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse adipose tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor D/Adipsin Monoclonal Antibody (Catalog # MAB5430) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Complement Factor D/Adipsin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Complement Factor D contains one peptidase S1 domain that cleaves an Arg‑Lys bond. There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse Complement Factor D shares 85% and 68% aa identity with rat and human Complement Factor D, respectively.

Entrez Gene IDs
1675 (Human); 11537 (Mouse); 54249 (Rat)
Alternate Names
Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC; PFD; Properdin factor DADIPSIN

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Citations for Mouse Complement Factor D/Adipsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Toll-like Receptor 2 Facilitates Oxidative Damage-Induced Retinal Degeneration
    Authors: K Mulfaul, E Ozaki, N Fernando, K Brennan, KR Chirco, E Connolly, C Greene, A Maminishki, RG Salomon, M Linetsky, R Natoli, RF Mullins, M Campbell, SL Doyle
    Cell Rep, 2020;30(7):2209-2224.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Fat-Produced Adipsin Regulates Inflammatory Arthritis
    Authors: Y Li, W Zou, JR Brestoff, N Rohatgi, X Wu, JP Atkinson, CA Harris, SL Teitelbaum
    Cell Rep, 2019;27(10):2809-2816.e3.
    Species: Transgenic Mouse
    Sample Types: Serum
    Applications: Western Blot


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