Detects mouse Contactin‑3 in direct ELISAS. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) Contactin-3 and no cross‑reactivity with recombinant mouse (rm) Contactin-1, -4, -6, rhContactin-2 or -5 is observed.
Monoclonal Rat IgG2A Clone # 524518
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse Contactin‑3 Glu20-Thr1003 Accession # Q07409
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Mouse Contactin‑3 by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Contactin‑3 Monoclonal Antibody (Catalog # MAB6644) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Contactin‑3 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
Detection of CNTN-3 in bEnd.3 mouse endothelioma cell line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Contactin‑3 Monoclonal Antibody (Catalog # MAB6644, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons, and olfactory granule cells. It likely mediates cell-cell interaction and may promote neurite outgrowth. The mouse Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region, and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG-like domains (aa 32-593), followed by four Fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, mouse Contactin-3 shares 92% and 96% aa identity with human and rat Contactin-3, respectively.
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