Mouse CRISP-1 Antibody (840017)

R&D Systems | Catalog # MAB4675

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 840017
View all CRISP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CRISP-1
Gln20-His244
Accession # Q03401

Specificity

Detects mouse CRISP-1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CRISP-1 Antibody (840017)

Proliferation Inhibited by CRISP‑1 and Neutralization by Mouse CRISP‑1 Antibody.

Proliferation Inhibited by CRISP‑1 and Neutralization by Mouse CRISP‑1 Antibody.

Recombinant Mouse CRISP-1 inhibits proliferation in the 3A-sub E human placenta cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation inhibited by Recombinant Mouse CRISP-1 (10 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CRISP-1 Monoclonal Antibody (Catalog # MAB4675). The ND50 is typically 10-20 µg/mL.
Detection of Mouse CRISP‑1 antibody by Western Blot.

Detection of Mouse CRISP‑1 by Western Blot.

Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CRISP-1 Monoclonal Antibody (Catalog # MAB4675) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CRISP-1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CRISP-1 antibody in Mouse Epididymis by Immunohistochemistry (IHC-Fr).

CRISP‑1 in Mouse Epididymis.

CRISP-1 was detected in perfusion fixed frozen sections of mouse epididymis using Rat Anti-Mouse CRISP-1 Monoclonal Antibody (Catalog # MAB4675) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse CRISP-1 Antibody (840017)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse epididymis

Western Blot

1 µg/mL
Sample: Mouse epididymis tissue

Neutralization

Measured by its ability to neutralize CRISP‑1-inhibited proliferation in the 3A‑sub E human placenta cell line. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 10 µg/mL Recombinant Mouse CRISP‑1.

Reviewed Applications

Read 1 review rated 5 using MAB4675 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRISP-1

Cysteine-rich Secretory Protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 29-32 kDa (26.4 kDa predicted) protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N-terminal SCP/CAP domain, a hinge region, and a Cys-rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP-1 in mouse and rat is CRISP-4 (5, 6). Mature mouse CRISP-1 shares 68% amino acid (aa) sequence identity with rat CRISP-1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP-2, -3, and -4, respectively. Rodent CRISP-1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7-9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP-1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP-1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13-15).

References

  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
  4. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
  6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
  7. Charest, N.J. et al. (1989) Endocrinology 125:942.
  8. Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
  9. Nixon, B. et al. (2006) Biol. Reprod. 74:275.
  10. Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
  11. Busso, D. et al. (2007) Biol. Reprod. 77:848.
  12. Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
  13. Haendler, B. et al. (1993) Endocrinology 133:192.
  14. Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
  15. Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.

Long Name

Cysteine-rich Secretory Protein 1

Alternate Names

AEGL1, CRISP1

Entrez Gene IDs

167 (Human); 11571 (Mouse); 64827 (Rat)

Gene Symbol

CRISP1

UniProt

Q03401

Additional CRISP-1 Products

Product Documents for Mouse CRISP-1 Antibody (840017)

Certificate of Analysis

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Product Specific Notices for Mouse CRISP-1 Antibody (840017)

For research use only

Citations for Mouse CRISP-1 Antibody (840017)

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  • Mouse CRISP-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Epididymis tissue
    Species: Mouse
    Verified Customer | Posted 10/27/2021
    Mouse CRISP-1 Antibody (840017) MAB4675

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