Mouse CRISP-1 Antibody

Name: Mouse CRISP-1 Antibody
Brand: R&D Systems
SKU: MAB4675-SP
Rating: 5 (1 reviews)

Catalog #: MAB4675
1 Citation 1 Review Datasheet / COA / SDS

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MAB4675
MAB4675-SP

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Proliferation Inhibited by CRISP‑1 and Neutralization by Mouse CRISP‑1 Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CRISP-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CRISP-1 in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 840017

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CRISP-1
Gln20-His244
Accession # Q03401

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize CRISP‑1-inhibited proliferation in the 3A‑sub E human placenta cell line. The Neutralization Dose (ND₅₀) is typically 10-20 µg/mL in the presence of 10 µg/mL Recombinant Mouse CRISP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Proliferation Inhibited by CRISP‑1 and Neutralization by Mouse CRISP‑1 Antibody. Recombinant Mouse CRISP-1 inhibits proliferation in the 3A-sub E human placenta cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation inhibited by Recombinant Mouse CRISP-1 (10 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CRISP-1 Monoclonal Antibody (Catalog # MAB4675). The ND₅₀ is typically 10-20 µg/mL.

Western Blot

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Detection of Mouse CRISP‑1 by Western Blot. Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CRISP-1 Monoclonal Antibody (Catalog # MAB4675) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CRISP-1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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CRISP‑1 in Mouse Epididymis. CRISP-1 was detected in perfusion fixed frozen sections of mouse epididymis using Rat Anti-Mouse CRISP-1 Monoclonal Antibody (Catalog # MAB4675) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-1

Cysteine-rich Secretory Protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 29-32 kDa (26.4 kDa predicted) protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N-terminal SCP/CAP domain, a hinge region, and a Cys-rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP-1 in mouse and rat is CRISP-4 (5, 6). Mature mouse CRISP-1 shares 68% amino acid (aa) sequence identity with rat CRISP-1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP-2, -3, and -4, respectively. Rodent CRISP-1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7-9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP-1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP-1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13-15).

References

Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
Charest, N.J. et al. (1989) Endocrinology 125:942.
Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
Nixon, B. et al. (2006) Biol. Reprod. 74:275.
Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
Busso, D. et al. (2007) Biol. Reprod. 77:848.
Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
Haendler, B. et al. (1993) Endocrinology 133:192.
Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.

Long Name

Cysteine-rich Secretory Protein 1

Entrez Gene IDs

167 (Human); 11571 (Mouse); 64827 (Rat)

Alternate Names

Acidic epididymal glycoprotein homolog; acidic epididymal glycoprotein-like 1; AEGL1; AEG-related protein; ARPAEG-like protein; CRISP1; CRISP-1; CRISP-1AEGL1HSCRISP1D; cysteine-rich secretory protein 1; cysteine-rich secretory protein-1 delta; HSCRISP1G; HUMARP

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Gametes and Fertilization

Citation for Mouse CRISP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CEP128 is involved in spermatogenesis in humans and mice
Authors: X Zhang, L Wang, Y Ma, Y Wang, H Liu, M Liu, L Qin, J Li, C Jiang, X Zhang, X Shan, Y Liu, J Li, Y Li, R Zheng, Y Sun, J Sun, X Leng, Y Liang, F Zhang, X Jiang, Y Yang, Y Shen
Nature Communications, 2022-03-16;13(1):1395.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot