Mouse CRISP-4 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CRISP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse CRISP-1, -2, and -3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CRISP-4
    Leu23-Lys250
    Accession # NP_084309
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CRISP‑4 by Western Blot. Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CRISP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5017) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CRISP‑4 at approximately 26-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRISP-4

CRISP‑4 (cysteine‑rich secretory protein 4) is a secreted, 31 kDa member of the CRISP family of proteins. In mouse, its expression is limited to the epididymis, seminal vesicle and vas deferens and it appears to bind to differentiating sperm. Mature mouse CRISP‑4 is 230 amino acids (aa) in length. It contains an SCP (sperm coating protein) region (aa 42‑178) followed by a C‑terminal CRISP domain (aa 196‑250). There appears to be an alternate start site four amino acids upstream of the standard start site. Over aa 22‑550, mouse CRISP‑4 shares 90% aa identity with rat CRISP‑4 and 64% aa identity with human CRISP‑1, the human ortholog to mouse CRISP‑4.

  • Long Name:
    Cysteine-rich Secretory Protein 4
  • Entrez Gene IDs:
    78081 (Mouse); 654517 (Rat)
  • Alternate Names:
    CRISP4; CRISP-4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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