|Detection of Mouse CRISP‑4 by Western Blot. Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CRISP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5017) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CRISP‑4 at approximately 26-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
CRISP‑4 (cysteine‑rich secretory protein 4) is a secreted, 31 kDa member of the CRISP family of proteins. In mouse, its expression is limited to the epididymis, seminal vesicle and vas deferens and it appears to bind to differentiating sperm. Mature mouse CRISP‑4 is 230 amino acids (aa) in length. It contains an SCP (sperm coating protein) region (aa 42‑178) followed by a C‑terminal CRISP domain (aa 196‑250). There appears to be an alternate start site four amino acids upstream of the standard start site. Over aa 22‑550, mouse CRISP‑4 shares 90% aa identity with rat CRISP‑4 and 64% aa identity with human CRISP‑1, the human ortholog to mouse CRISP‑4.
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