Mouse Cryptic Antibody Summary
Accession # P97766
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cryptic in D3 Mouse Cell Line. Cryptic was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse Cryptic Monoclonal Antibody (Catalog # MAB18401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cryptic, also known as CFC-1, is a member of the Epidermal Growth Factor-Cripto/FRL-1/Cryptic (EGF-CFC) family of signaling proteins. It is expressed during mesoderm differentation and is necessary for determining left-right asymmetry. Cryptic functions as a co-receptor that regulates signaling by some TGF-beta superfamily members.
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