Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody Summary
Arg20-Lys96
Accession # P12850
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Applications
Mouse CXCL1/KC Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC‑1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody. Recombinant Mouse CXCL1/GROa/KC/ CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453). The ND50 is typically 0.05-0.25 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1
CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.
Product Datasheets
Citations for Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
51
Citations: Showing 1 - 10
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PD-L1 negatively regulates antifungal immunity by inhibiting neutrophil release from bone marrow
Authors: Y Yu, RR Wang, NJ Miao, JJ Tang, YW Zhang, XR Lu, PY Yan, J Wang, XM Jia
Nature Communications, 2022;13(1):6857.
Species: Mouse
Sample Types: In Vivo
Applications: IHC -
KLK6/PAR1 Axis Promotes Tumor Growth and Metastasis by Regulating Cross-Talk between Tumor Cells and Macrophages
Authors: YS Hwang, HJ Cho, ES Park, J Lim, HR Yoon, JT Kim, SR Yoon, H Jung, YK Choe, YH Kim, CH Lee, YT Kwon, BY Kim, HG Lee
Cells, 2022;11(24):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer
Authors: J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi
Cancer Research, 2022;0(0):OF1-OF14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Enteric glial adenosine 2B receptor signaling mediates persistent epithelial barrier dysfunction following acute DSS colitis
Authors: V Grubiši?, V Bali, DE Fried, HK Eltzschig, SC Robson, MS Mazei-Robi, BD Gulbransen
Mucosal Immunology, 2022;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis by dysregulating redox homeostasis
Authors: D Kang, J Lee, J Jung, BA Carlson, MJ Chang, CB Chang, SB Kang, BC Lee, VN Gladyshev, DL Hatfield, BJ Lee, JH Kim
Nature Communications, 2022;13(1):779.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
GSDMD contributes to host defence against Staphylococcus aureus skin infection by suppressing the Cxcl1-Cxcr2 axis
Authors: ZZ Liu, YJ Yang, FH Zhou, K Ma, XQ Lin, SQ Yan, Y Gao, W Chen
Veterinary research, 2021;52(1):71.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Keratinocyte transglutaminase 2 promotes CCR6+ &gamma&deltaT-cell recruitment by upregulating CCL20 in psoriatic inflammation
Authors: JW Shin, MA Kwon, J Hwang, SJ Lee, JH Lee, HJ Kim, KB Lee, SJ Lee, EM Jeong, JH Chung, IG Kim
Cell Death Dis, 2020;11(4):301.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Local microvascular leakage promotes trafficking of activated neutrophils to remote organs
Authors: C Owen-Woods, R Joulia, A Barkaway, L Rolas, B Ma, AF Nottebaum, KP Arkill, M Stein, T Girbl, M Golding, DO Bates, D Vestweber, MB Voisin, S Nourshargh
J. Clin. Invest., 2020;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Neutralization -
Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1
Authors: J Sellau, M Groneberg, H Fehling, T Thye, S Hoenow, C Marggraff, M Weskamm, C Hansen, S Stanelle-B, S Kuehl, J Noll, V Wolf, NG Metwally, SH Hagen, C Dorn, J Wernecke, H Ittrich, E Tannich, T Jacobs, I Bruchhaus, M Altfeld, H Lotter
Nat Commun, 2020;11(1):3459.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Excessive neutrophil levels in the lung underlie the age-associated increase in influenza mortality
Authors: U Kulkarni, RL Zemans, CA Smith, SC Wood, JC Deng, DR Goldstein
Mucosal Immunol, 2019;12(2):545-554.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Neutralization -
Deletion of C-C Motif Chemokine Ligand 5 Worsens Invariant Natural Killer T-Cell Mediated Hepatitis via Compensatory Up-regulation of CXCR2 Related Chemokine Activity
Authors: L Chen, J Gu, Y Qian, M Li, Y Qian, M Xu, J Li, Y Wen, L Xia, J Li, Q Xia, X Kong, H Wu
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Targeting inflammatory monocytes in sepsis-associated encephalopathy and long-term cognitive impairment
Authors: G Andonegui, EL Zelinski, CL Schubert, D Knight, LA Craig, BW Winston, SC Spanswick, B Petri, CN Jenne, JC Sutherland, R Nguyen, N Jayawarden, MM Kelly, CJ Doig, RJ Sutherland, P Kubes
JCI Insight, 2018;3(9):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
Cell. Physiol. Biochem., 2017;42(5):1961-1972.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Capture) -
Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection
Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel
J. Immunol., 2017;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype
PLoS ONE, 2016;11(8):e0159986.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity
Sci Rep, 2016;6(0):29994.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression
Authors: L Seifert, G Werba, S Tiwari, NN Giao Ly, S Alothman, D Alqunaibit, A Avanzi, R Barilla, D Daley, SH Greco, A Torres-Her, M Pergamo, A Ochi, CP Zambirinis, M Pansari, M Rendon, D Tippens, M Hundeyin, VR Mani, C Hajdu, D Engle, G Miller
Nature, 2016;532(7598):245-9.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Induction of the MCP chemokine cluster cascade in the periphery by cancer cell-derived CCL3
Authors: Elena Farmaki
Cancer Lett, 2016;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
Authors: Clara Jana-Lui Busch
Hepatology, 2016;0(0):.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
Applications: ELISA Development (Capture) -
Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
MicroRNA-146a provides feedback regulation of lyme arthritis but not carditis during infection with Borrelia burgdorferi.
Authors: Lochhead R, Ma Y, Zachary J, Baltimore D, Zhao J, Weis J, O'Connell R, Weis J
PLoS Pathog, 2014;10(6):e1004212.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development -
CXCL1 but not IL-6 is required for recurrent herpetic stromal keratitis.
Authors: West D, Del Rosso C, Yin X, Stuart P
J Immunol, 2014;192(4):1762-7.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
J Immunol, 2013;190(10):5329-36.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
J Immunol, 2013;190(10):5178-86.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development (Capture) -
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development -
CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
Authors: Roy M, Richard JF, Dumas A, Vallieres L
J Neuroinflammation, 2012;9(0):18.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
J. Clin. Invest., 2011;121(11):4446-61.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
Am. J. Pathol., 2011;179(6):2730-9.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development -
Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
Authors: Chou RC, Kim ND, Sadik CD
Immunity, 2010;33(2):266-78.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
Authors: Summers SA, van der Veen BS, O'Sullivan KM
Kidney Int., 2010;78(12):1263-74.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
Microbes Infect., 2009;11(4):524-30.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Interleukin 17 sustains rather than induces inflammation.
Authors: Maione F, Paschalidis N, Mascolo N, Dufton N, Perretti M, D'Acquisto F
Biochem. Pharmacol., 2009;77(5):878-87.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology, 2008;125(2):272-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways.
Authors: De Filippo K, Henderson RB, Laschinger M, Hogg N
J. Immunol., 2008;180(6):4308-15.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
Blood, 2008;111(7):3343-54.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Keratocan and lumican regulate neutrophil infiltration and corneal clarity in lipopolysaccharide-induced keratitis by direct interaction with CXCL1.
Authors: Carlson EC, Lin M, Liu CY, Kao WW, Perez VL, Pearlman E
J. Biol. Chem., 2007;282(49):35502-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
Blood, 2007;111(1):42-9.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
Eur. J. Pharmacol., 2007;573(1):221-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infect. Immun., 2006;74(12):6730-8.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development -
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
J. Immunol., 2006;177(1):612-20.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development -
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
J. Immunol., 2006;177(12):8650-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
J. Immunol., 2006;177(3):1967-74.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development -
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
Eur. J. Immunol., 2006;36(8):2025-34.
Species: Mouse
Sample Types: Tissue Secretion
Applications: ELISA Development -
Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
Inflamm. Res., 2006;55(11):457-64.
Species: Mouse
Sample Types: Synovial Fluid
Applications: ELISA Development -
Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
J. Immunol., 2005;175(4):2589-97.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development -
Selective macrophage suppression during sepsis.
Authors: Ellaban E, Bolgos G, Remick D
Cell. Immunol., 2004;231(1):103-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Regulatory effects of eotaxin on acute lung inflammatory injury.
Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
J. Immunol., 2001;166(8):5208-18.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development -
Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
J. Immunol., 2001;166(2):1233-40.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
Immunity, 0;43(5):945-58.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)
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