Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody

(36 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. Shows 100% cross‑reactivity with recombinant human (rh) CXCL2, less than 40% cross‑reactivity with recombinant rat (rr) CINC-1, and no cross‑reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse CXCL6.
  • Source
    Monoclonal Rat IgG2A Clone # 48415
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
    Arg20-Lys96
    Accession # P12850
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
    • Mouse CXCL1/KC Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog # MAB453)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (Catalog # BAF453)
  • ELISA Standard
     
    Recombinant Mouse CXCL1/KC Protein (Catalog # 453-KC)
  • Neutralization
    Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC‑1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody. Recombinant Mouse CXCL1/GRO alpha /KC/
CINC‑1 aa 20‑96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453). The ND50 is typically
0.05‑0.25 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1
CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.
  • Entrez Gene IDs:
    2919 (Human); 14825 (Mouse); 81503 (Rat)
  • Alternate Names:
    CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  2. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression
    Authors: L Seifert, G Werba, S Tiwari, NN Giao Ly, S Alothman, D Alqunaibit, A Avanzi, R Barilla, D Daley, SH Greco, A Torres-Her, M Pergamo, A Ochi, CP Zambirinis, M Pansari, M Rendon, D Tippens, M Hundeyin, VR Mani, C Hajdu, D Engle, G Miller
    Nature, 2016;532(7598):245-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity
    Sci Rep, 2016;6(0):29994.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype
    PLoS ONE, 2016;11(8):e0159986.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. MicroRNA-146a provides feedback regulation of lyme arthritis but not carditis during infection with Borrelia burgdorferi.
    Authors: Lochhead R, Ma Y, Zachary J, Baltimore D, Zhao J, Weis J, O'Connell R, Weis J
    PLoS Pathog, 2014;10(6):e1004212.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  7. CXCL1 but not IL-6 is required for recurrent herpetic stromal keratitis.
    Authors: West D, Del Rosso C, Yin X, Stuart P
    J Immunol, 2014;192(4):1762-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  8. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013;190(10):5178-86.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Capture
  9. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  10. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
    Authors: Roy M, Richard JF, Dumas A, Vallieres L
    J Neuroinflammation, 2012;9(0):18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  11. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  12. Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
    Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
    J. Clin. Invest., 2011;121(11):4446-61.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011;179(6):2730-9.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  14. Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
    Authors: Summers SA, van der Veen BS, O'Sullivan KM
    Kidney Int., 2010;78(12):1263-74.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  15. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010;33(2):266-78.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  17. Interleukin 17 sustains rather than induces inflammation.
    Authors: Maione F, Paschalidis N, Mascolo N, Dufton N, Perretti M, D'Acquisto F
    Biochem. Pharmacol., 2009;77(5):878-87.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  19. Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways.
    Authors: De Filippo K, Henderson RB, Laschinger M, Hogg N
    J. Immunol., 2008;180(6):4308-15.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  20. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  21. HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
    Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
    Blood, 2008;111(7):3343-54.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. Keratocan and lumican regulate neutrophil infiltration and corneal clarity in lipopolysaccharide-induced keratitis by direct interaction with CXCL1.
    Authors: Carlson EC, Lin M, Liu CY, Kao WW, Perez VL, Pearlman E
    J. Biol. Chem., 2007;282(49):35502-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  23. TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
    Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
    Eur. J. Pharmacol., 2007;573(1):221-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  24. The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
    Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
    Blood, 2007;111(1):42-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  25. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006;177(1):612-20.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  26. Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
    Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
    Inflamm. Res., 2006;55(11):457-64.
    Species: Mouse
    Sample Type: Synovial Fluid
    Application: ELISA Development
  27. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  28. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006;177(12):8650-7.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  29. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006;36(8):2025-34.
    Species: Mouse
    Sample Type: Tissue Secretion
    Application: ELISA Development
  30. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  31. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005;175(4):2589-97.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  32. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  33. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  34. Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
    Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
    J. Immunol., 2001;166(2):1233-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  35. Regulatory effects of eotaxin on acute lung inflammatory injury.
    Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
    J. Immunol., 2001;166(8):5208-18.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  36. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 0;43(5):945-58.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
Expand to show all 36 Citations
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Secondary Antibodies
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Goat Anti-Rat IgG Biotinylated Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Average Rating: 5 (Based on 1 review)

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ELISA Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody MAB453
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ELISA Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody MAB453
ELISA: Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody [MAB453]

Summary

ApplicationELISA
Sample TestedPurified Recombinant Mouse CXCL1/GROalpha/KC/CINC1 aa 2096

Other Experimental Details

Other Experimental Details50ul of KC Capture antibody (2ug/mL) was coated per well of a 96 well plate at 4 degrees overnight. Purified KC standard was diluted (6 fold) and assayed to ensure the capture antibody worked. Mouse CXCL1/GRO/KC/CINC1 Biotinylated Antibody was added followed by the addition of substrate solution to measure KC concentration. The standard curve looked great as was determined by calculating the R- squared value. Highly recommend doing ELISA's using the following KC capture antibody.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 100ng/well of a 96 well plate

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