Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody

  (39 citations)
(2 Reviews)
    
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Product Details
Citations (39)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. Shows 100% cross‑reactivity with recombinant human (rh) CXCL2, less than 40% cross‑reactivity with recombinant rat (rr) CINC-1, and no cross‑reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse CXCL6.
  • Source
    Monoclonal Rat IgG2A Clone # 48415
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
    Arg20-Lys96
    Accession # P12850
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
    • Mouse CXCL1/KC Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog # MAB453 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (Catalog # BAF453 )
  • ELISA Standard
     
    Recombinant Mouse CXCL1/KC Protein (Catalog # 453-KC )
  • Neutralization
    Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC‑1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody. Recombinant Mouse CXCL1/GRO alpha /KC/
CINC‑1 aa 20‑96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453). The ND50 is typically
0.05‑0.25 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1
CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.
  • Entrez Gene IDs:
    2919 (Human); 14825 (Mouse); 81503 (Rat)
  • Alternate Names:
    CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection
    Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  3. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
    Authors: Clara Jana-Lui Busch
    Hepatology, 2016;0(0):.
    Species: Mouse
    Sample Type: Peritoneal Lavage Fluid
    Application: ELISA Capture
  4. Induction of the MCP chemokine cluster cascade in the periphery by cancer cell-derived CCL3
    Authors: Elena Farmaki
    Cancer Lett, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Neut
  5. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression
    Authors: L Seifert, G Werba, S Tiwari, NN Giao Ly, S Alothman, D Alqunaibit, A Avanzi, R Barilla, D Daley, SH Greco, A Torres-Her, M Pergamo, A Ochi, CP Zambirinis, M Pansari, M Rendon, D Tippens, M Hundeyin, VR Mani, C Hajdu, D Engle, G Miller
    Nature, 2016;532(7598):245-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity
    Sci Rep, 2016;6(0):29994.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  7. Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype
    PLoS ONE, 2016;11(8):e0159986.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  9. MicroRNA-146a provides feedback regulation of lyme arthritis but not carditis during infection with Borrelia burgdorferi.
    Authors: Lochhead R, Ma Y, Zachary J, Baltimore D, Zhao J, Weis J, O'Connell R, Weis J
    PLoS Pathog, 2014;10(6):e1004212.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  10. CXCL1 but not IL-6 is required for recurrent herpetic stromal keratitis.
    Authors: West D, Del Rosso C, Yin X, Stuart P
    J Immunol, 2014;192(4):1762-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  11. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013;190(10):5178-86.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Capture
  12. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  13. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
    Authors: Roy M, Richard JF, Dumas A, Vallieres L
    J Neuroinflammation, 2012;9(0):18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  14. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  15. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011;179(6):2730-9.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  16. Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
    Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
    J. Clin. Invest., 2011;121(11):4446-61.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  17. Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis.
    Authors: Summers SA, van der Veen BS, O'Sullivan KM
    Kidney Int., 2010;78(12):1263-74.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010;33(2):266-78.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  19. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  20. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  21. Interleukin 17 sustains rather than induces inflammation.
    Authors: Maione F, Paschalidis N, Mascolo N, Dufton N, Perretti M, D'Acquisto F
    Biochem. Pharmacol., 2009;77(5):878-87.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  23. Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways.
    Authors: De Filippo K, Henderson RB, Laschinger M, Hogg N
    J. Immunol., 2008;180(6):4308-15.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  24. HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
    Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
    Blood, 2008;111(7):3343-54.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  25. Keratocan and lumican regulate neutrophil infiltration and corneal clarity in lipopolysaccharide-induced keratitis by direct interaction with CXCL1.
    Authors: Carlson EC, Lin M, Liu CY, Kao WW, Perez VL, Pearlman E
    J. Biol. Chem., 2007;282(49):35502-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  26. TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
    Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
    Eur. J. Pharmacol., 2007;573(1):221-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  27. The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
    Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
    Blood, 2007;111(1):42-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  28. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006;177(1):612-20.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  29. Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
    Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
    Inflamm. Res., 2006;55(11):457-64.
    Species: Mouse
    Sample Type: Synovial Fluid
    Application: ELISA Development
  30. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006;36(8):2025-34.
    Species: Mouse
    Sample Type: Tissue Secretion
    Application: ELISA Development
  31. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  32. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  33. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006;177(12):8650-7.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  34. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  35. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005;175(4):2589-97.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  36. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  37. Regulatory effects of eotaxin on acute lung inflammatory injury.
    Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
    J. Immunol., 2001;166(8):5208-18.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  38. Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
    Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
    J. Immunol., 2001;166(2):1233-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  39. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 0;43(5):945-58.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
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Isotype Controls
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Secondary Antibodies
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Average Rating: 5 (Based on 2 reviews)

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We have 2 review tested in 1 species: Mouse.
We have 2 review tested in 1 application: ELISA.

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Images Ratings Applications Species Reviewed By Date Details
ELISA Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody MAB453 Array MAB453
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Excellent
 ELISA Mouse Anonymous 01/05/2018
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ELISA Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody MAB453
ELISA: Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody [MAB453].

Summary

Application ELISA
Sample Tested Serum and Plasma
Species Mouse

Other Experimental Details

Other Experimental Details This antibody was used with the polyclonal BAF453 to build an ELISA to measure KC in mouse samples.
ELISA Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody MAB453 Array MAB453
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Excellent
 ELISA Mouse Anonymous 10/26/2015
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Image Details
ELISA Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody MAB453
ELISA: Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody [MAB453].

Summary

Application ELISA
Sample Tested Purified Recombinant Mouse CXCL1/GROalpha/KC/CINC1 aa 2096
Species Mouse

Other Experimental Details

Other Experimental Details 50ul of KC Capture antibody (2ug/mL) was coated per well of a 96 well plate at 4 degrees overnight. Purified KC standard was diluted (6 fold) and assayed to ensure the capture antibody worked. Mouse CXCL1/GRO/KC/CINC1 Biotinylated Antibody was added followed by the addition of substrate solution to measure KC concentration. The standard curve looked great as was determined by calculating the R- squared value. Highly recommend doing ELISA's using the following KC capture antibody.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 100ng/well of a 96 well plate

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