Mouse CXCL16 Antibody

Catalog # Availability Size / Price Qty
AF503
AF503-SP
Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (2)

Mouse CXCL16 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL16 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL16, rhFractalkine, recombinant mouse Fractalkine, and recombinant rat Fractalkine is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX)
Flow Cytometry
2.5 µg/106 cells
Raw264.7 mouse monocyte/macrophage cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CXCL16-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nature Immunol. 1:298. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse CXCL16 Chemokine Domain.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. View Larger

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF503). The ND50 is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL16

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine and CXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa residue signal peptide, an 88 aa residue chemokine domain, an 87 aa residue mucin-like spacer region, a 22 aa residue transmembrane domain, and a 23 aa residue cytoplasmic tail. Mouse and human CXCL16 share 49% overall aa identity and 70% similarity in the chemokine domains. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and by cells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some nonlymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6/Bonzo (STRL33 and TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Entrez Gene IDs
58191 (Human); 66102 (Mouse)
Alternate Names
chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16

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Citations for Mouse CXCL16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitors
    Authors: A Vorontsova, TJ Cooper, J Haj-Shomal, M Benguigui, S Levin, B Manobla, R Menachem, M Timaner, Z Raviv, Y Shaked
    Oncogene, 2023;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Distinct hepatic immunological patterns are associated with the progression or inhibition of hepatocellular carcinoma
    Authors: F Mirshahi, HF Aqbi, M Isbell, SH Manjili, C Guo, M Saneshaw, M Dozmorov, A Khosla, K Wack, OM Carrasco-Z, MO Idowu, XY Wang, AJ Sanyal, MH Manjili, D Bandyopadh
    Cell Reports, 2022;38(9):110454.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. IL-33 modulates inflammatory brain injury but exacerbates systemic immunosuppression following ischemic stroke
    Authors: SR Zhang, M Piepke, HX Chu, BR Broughton, R Shim, CH Wong, S Lee, MA Evans, A Vinh, S Sakkal, TV Arumugam, T Magnus, S Huber, M Gelderblom, GR Drummond, CG Sobey, HA Kim
    JCI Insight, 2018;3(18):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
    Authors: Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
    Arthritis Rheum., 2007;56(3):949-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.
    Authors: Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
    J. Leukoc. Biol., 2007;81(1):176-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
    Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
    J. Immunol., 2007;179(10):7166-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006;54(8):2402-14.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Neutralization

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Mouse CXCL16 Antibody

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Mouse CXCL16 Antibody
By Luiz Alexandre on 07/19/2018
Application: WB Sample Tested: Liver tissue Species: Mouse

Western blot of liver tissue from a animal model of inflammatory liver disease shows increased expression of CXCL16 protein (RIGHT), relative to control mice (LEFT).


Mouse CXCL16 Antibody
By Ravikumar Muthuswamy on 08/30/2017
Application: ICC/IF Sample Tested: Ovarian cell line Species: Mouse