Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Xenograft

Applications

Validated:

Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CXCL16 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2

Specificity

Detects mouse CXCL16 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL16, rhFractalkine, recombinant mouse Fractalkine, and recombinant rat Fractalkine is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL16 Antibody

Detection of CXCL16 in Raw 264.7 cells by Flow Cytometry

Raw 264.7 cells were stained with Goat Anti-Mouse CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # af503, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.

Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF503). The ND50 is typically 0.1-0.4 µg/mL.

Applications for Mouse CXCL16 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Raw264.7 mouse monocyte/macrophage cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX)

Neutralization

Measured by its ability to neutralize CXCL16-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nature Immunol. 1:298. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse CXCL16 Chemokine Domain.

Reviewed Applications

Read 2 reviews rated 5 using AF503 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL16

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine and CXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa residue signal peptide, an 88 aa residue chemokine domain, an 87 aa residue mucin-like spacer region, a 22 aa residue transmembrane domain, and a 23 aa residue cytoplasmic tail. Mouse and human CXCL16 share 49% overall aa identity and 70% similarity in the chemokine domains. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and by cells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some nonlymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6/Bonzo (STRL33 and TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Alternate Names

CXCL16

Entrez Gene IDs

58191 (Human); 66102 (Mouse)

Gene Symbol

CXCL16

UniProt

Q8BSU2

Additional CXCL16 Products

Product Documents for Mouse CXCL16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCL16 Antibody

For research use only

Citations for Mouse CXCL16 Antibody

Customer Reviews for Mouse CXCL16 Antibody (2)

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Customer Images

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  • Mouse CXCL16 Antibody
    Name: Luiz Alexandre
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 07/19/2018
    Western blot of liver tissue from a animal model of inflammatory liver disease shows increased expression of CXCL16 protein (RIGHT), relative to control mice (LEFT).
    Mouse CXCL16 Antibody AF503
  • Mouse CXCL16 Antibody
    Name: Ravikumar Muthuswamy
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Ovarian cell line
    Species: Mouse
    Verified Customer | Posted 08/30/2017
    Mouse CXCL16 Antibody AF503

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