CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
Mouse CXCL2/MIP-2 Quantikine ELISA Kit
R&D Systems | Catalog # MM200
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
1.5 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum)
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Product Summary for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
The Quantikine Mouse MIP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MIP-2 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse MIP-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse MIP-2 accurately. Results obtained using natural mouse MIP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MIP-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse MIP-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 17.7 | 53.9 | 363 | 16.9 | 52.1 | 346 |
| Standard Deviation | 0.5 | 1.3 | 7.3 | 1.0 | 2.3 | 15.4 |
| CV% | 2.8 | 2.4 | 2.0 | 5.9 | 4.4 | 4.5 |
Recovery for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
The recovery of mouse MIP-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=10) | 96 | 84-110 |
| Serum (n=14) | 100 | 94-116 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse MIP-2 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
Mouse CXCL2/GRO beta/MIP-2/CINC-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Alternate Names
CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2
Gene Symbol
CXCL2
Additional CXCL2/GRO beta/MIP-2/CINC-3 Products
Product Documents for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse CXCL2/MIP-2 Quantikine ELISA Kit
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Sample Tested: Mouse lung BALFVerified Customer | Posted 12/04/2017Result has been published in 10.1371/journal.pone.0058258
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Sample Tested: Lung tissueVerified Customer | Posted 08/04/2017
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Protocols
View specific protocols for Mouse CXCL2/MIP-2 Quantikine ELISA Kit (MM200):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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