Mouse CXCL7/Thymus Chemokine-1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL7/Thymus Chemokine‑1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, recombinant mouse (rm) CXCL1, 4, 6, 9, 10, 11, 12, 13, 15, 16, recombinant rat (rr) CXCL1, 2, 3, 5, recombinant porcine CXCL8, or recombinant cotton rat CXCL10 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 159742
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCL7/Thymus Chemokine‑1
    Lys40-Tyr113
    Accession # Q9EQI5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK) under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Mouse CXCL7/Thymus Chemokine‑1 Antibody. Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109 (40 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL7/Thymus Chemokine‑1 Monoclonal Antibody (Catalog # MAB1091). The ND50 is typically 2-8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/Thymus Chemokine-1
CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid precursor protein that shares 72% amino acid sequence identity with rat CXCL7.
  • Entrez Gene IDs:
    57349 (Mouse); 246358 (Rat)
  • Alternate Names:
    Thymus Chemokine1; Thymus Chemokine-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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