Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 159742
View all CXCL7/Thymus Chemokine-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL7/Thymus Chemokine‑1
Lys40-Tyr113
Accession # Q9EQI5

Specificity

Detects mouse CXCL7/Thymus Chemokine‑1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, recombinant mouse (rm) CXCL1, 4, 6, 9, 10, 11, 12, 13, 15, 16, recombinant rat (rr) CXCL1, 2, 3, 5, recombinant porcine CXCL8, or recombinant cotton rat CXCL10 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL7/Thymus Chemokine‑1 Antibody

Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Mouse CXCL7/Thymus Chemokine‑1 Antibody.

Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Mouse CXCL7/Thymus Chemokine‑1 Antibody.

Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109 (40 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL7/Thymus Chemokine‑1 Monoclonal Antibody (Catalog # MAB1091). The ND50 is typically 2-8 µg/mL.

Applications for Mouse CXCL7/Thymus Chemokine‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid precursor protein that shares 72% amino acid sequence identity with rat CXCL7.

Alternate Names

Thymus Chemokine1

Entrez Gene IDs

57349 (Mouse); 246358 (Rat)

Gene Symbol

PPBP

UniProt

Q9EQI5

Additional CXCL7/Thymus Chemokine-1 Products

Product Documents for Mouse CXCL7/Thymus Chemokine‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCL7/Thymus Chemokine‑1 Antibody

For research use only

Citations for Mouse CXCL7/Thymus Chemokine‑1 Antibody

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