Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 614641
View all CXCR5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCR5
Met1-Pro57
Accession # Q04683

Specificity

Detects recombinant mouse (rm) CXCR5 in direct ELISAs. In this format, no cross-reactivity with rmCXCR7 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CXCR5 APC‑conjugated Antibody

Detection of CXCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR5 antibody in Mouse T Follicular Helper Cells antibody by Flow Cytometry.

Detection of CXCR5 in Mouse T Follicular Helper Cells by Flow Cytometry.

Mouse T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB6198A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Detection of Human CXCR5 by Flow Cytometry

Detection of Human CXCR5 by Flow Cytometry

The percentages of CD4+CXCR5+ICOS5+ T cells in peripheral blood of patients with T1D.Peripheral blood mononuclear cells (PMBCs) from T1D patients (n = 54) and healthy controls (n = 31) were stained with labelled antibodies as described in Methods. A. Representative dot plots of CD4+CXCR5+ICOS5+ T cells in different groups of samples. Values in the upper right quadrant correspond to the percentages of CD4+CXCR5+ICOS5+ T cells. At least about 50,000 events were analyzed for each sample. B. CD4+CXCR5+ICOS5+ T cells were compared between T1D patients and healthy controls. Each data point represents an individual subject. The bars indicate the mean values. Student's unpaired t test was performed. ***, P<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24278195), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CXCR5 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes and T follicular helper cells

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

Alternate Names

BLR1, CD185, CXCR5

Entrez Gene IDs

643 (Human); 12145 (Mouse); 29363 (Rat)

Gene Symbol

CXCR5

UniProt

Q04683

Additional CXCR5 Products

Product Documents for Mouse CXCR5 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CXCR5 APC‑conjugated Antibody

For research use only

Citations for Mouse CXCR5 APC‑conjugated Antibody

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