Mouse CXCR5 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects recombinant mouse CXCR5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CXCR7 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 614641
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCR5
    Met1-Pro57
    Accession # Q04683
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-reported
    This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CXCR5 in A20 Mouse Cell Line. CXCR5 was detected in immersion fixed A20 mouse B cell lymphoma cell line using Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Detection of CXCR5 in T Follicular Helper Cells by Flow Cytometry. T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR5
CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.
  • Entrez Gene IDs:
    643 (Human); 12145 (Mouse); 29363 (Rat)
  • Alternate Names:
    BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15
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