Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-reported

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 614641
View all CXCR5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCR5
Met1-Pro57
Accession # Q04683

Specificity

Detects recombinant mouse CXCR5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CXCR7 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CXCR5 Antibody

CXCR5 antibody in A20 Mouse Cell Line by Immunocytochemistry (ICC).

CXCR5 in A20 Mouse Cell Line.

CXCR5 was detected in immersion fixed A20 mouse B cell lymphoma cell line using Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CXCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Detection of CXCR5 antibody in T Follicular Helper Cells antibody by Flow Cytometry.

Detection of CXCR5 in T Follicular Helper Cells by Flow Cytometry.

T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Mouse CXCR5 Antibody

Application
Recommended Usage

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample:   Mouse splenocytes and T follicular helper cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes and immersion fixed A20 mouse B cell lymphoma cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR5

CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.

Alternate Names

BLR1, CD185, CXCR5

Entrez Gene IDs

643 (Human); 12145 (Mouse); 29363 (Rat)

Gene Symbol

CXCR5

UniProt

Q04683

Additional CXCR5 Products

Product Documents for Mouse CXCR5 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CXCR5 Antibody

For research use only

Citations for Mouse CXCR5 Antibody

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Protocols

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