Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cystatin C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C (R & D Systems, Catalog # 1238-PI)
Ala21-Ala140
Accession # P21460

Specificity

Detects mouse and human Cystatin C in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human Cystatin D is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Cystatin C Antibody

Detection of Mouse Cystatin C antibody by Western Blot.

Detection of Mouse Cystatin C by Western Blot.

Western blot shows lysates of mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cystatin C antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr).

Cystatin C in Mouse Spleen.

Cystatin C was detected in immersion fixed frozen sections of mouse spleen using Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Cystatin C by Simple WesternTM.

Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for Cystatin C at approximately 19 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Cystatin C Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (caudate putamen) and immersion fixed frozen sections of mouse spleen

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cystatin C (Catalog # 1238-PI), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Western Blot

1 µg/mL
Sample: Mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line

Mouse Cystatin C Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Cystatin C Biotinylated Antibody (Catalog # BAF1238)
  • Standard: Recombinant Mouse Cystatin C Protein, CF (Catalog # 1238-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF1238 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin C

Cystatin C is a member of family 2 of the cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). All mouse tissues analyzed expressed Cystatin C, with relative levels similar to those of rat and human tissues. For all three species, brain and liver had the highest and lowest levels of Cystatin C, respectively, whereas kidney, spleen and muscle had the levels in between (4). The high degree of similarity in distribution and functional properties for mouse, rat and human Cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy (4).

References

  1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
  2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
  3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  4. Hakansson, K. et al. (1996) Comp. Biochem. Physiol. 114B:303.

Alternate Names

ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Gene Symbol

CST3

UniProt

P21460

Additional Cystatin C Products

Product Documents for Mouse Cystatin C Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Cystatin C Antibody

For research use only

Citations for Mouse Cystatin C Antibody

Customer Reviews for Mouse Cystatin C Antibody (1)

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    Application: Flow Cytometry
    Sample Tested: CHO Chinese hamster ovary cell line
    Species: Mouse
    Verified Customer | Posted 08/30/2023
    Mouse Cystatin C Antibody AF1238

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Protocols

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