Mouse Cystatin C Antibody

Catalog # Availability Size / Price Qty
AF1238
AF1238-SP
Detection of Mouse Cystatin C by Western Blot.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Mouse Cystatin C Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse and human Cystatin C in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human Cystatin D is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C (R & D Systems, Catalog # 1238-PI)
Ala21-Ala140
Accession # P21460
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Cystatin C (Catalog # 1238-PI), see our available Western blot detection antibodies

Mouse Cystatin C Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Cystatin C Biotinylated Antibody (Catalog # BAF1238)

Standard: Recombinant Mouse Cystatin C Protein, CF (Catalog # 1238-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Cystatin C antibody by Western Blot. View Larger

Detection of Mouse Cystatin C by Western Blot. Western blot shows lysates of mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cystatin C antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

Cystatin C in Mouse Spleen. Cystatin C was detected in immersion fixed frozen sections of mouse spleen using Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western View Larger

Detection of Mouse Cystatin C by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for Cystatin C at approximately 19 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1238). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 of the cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). All mouse tissues analyzed expressed Cystatin C, with relative levels similar to those of rat and human tissues. For all three species, brain and liver had the highest and lowest levels of Cystatin C, respectively, whereas kidney, spleen and muscle had the levels in between (4). The high degree of similarity in distribution and functional properties for mouse, rat and human Cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy (4).

References
  1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
  2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
  3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  4. Hakansson, K. et al. (1996) Comp. Biochem. Physiol. 114B:303.
Entrez Gene IDs
1471 (Human); 13010 (Mouse); 25307 (Rat)
Alternate Names
ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin

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Citations for Mouse Cystatin C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution
    Authors: T Masuda, R Sankowski, O Staszewski, C Böttcher, L Amann, C Scheiwe, S Nessler, P Kunz, G van Loo, VA Coenen, PC Reinacher, A Michel, U Sure, R Gold, J Priller, C Stadelmann, M Prinz
    Nature, 2019-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Megalin dependent urinary cystatin C excretion in ischemic kidney injury in rats
    Authors: D Jensen, C Kierulf-La, MLV Kristensen, R Nørregaard, K Weyer, R Nielsen, EI Christense, H Birn
    PLoS ONE, 2017-06-02;12(6):e0178796.
    Species: Mouse
    Sample Types: Urine
    Applications: Western Blot
  3. Peripheral role of cathepsin S in Th1 cell-dependent transition of nerve injury-induced acute pain to a chronic pain state.
    Authors: Zhang X, Wu Z, Hayashi Y, Okada R, Nakanishi H
    J Neurosci, 2014-02-19;34(8):3013-22.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  4. Urinary clusterin, cystatin C, beta2-microglobulin and total protein as markers to detect drug-induced kidney injury.
    Authors: Dieterle F, Perentes E, Cordier A, Roth DR, Verdes P, Grenet O, Pantano S, Moulin P, Wahl D, Mahl A, End P, Staedtler F, Legay F, Carl K, Laurie D, Chibout SD, Vonderscher J, Maurer G
    Nat. Biotechnol., 2010-05-01;28(5):463-9.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development
  5. pH-dependent and dynamic interactions of cystatin C with heparan sulfate
    Authors: Xiaoxiao Zhang, Xinyue Liu, Guowei Su, Miaomiao Li, Jian Liu, Chunyu Wang et al.
    Communications Biology
  6. Osteopontin Is a Blood Biomarker for Microglial Activation and Brain Injury in Experimental Hypoxic-Ischemic Encephalopathy
    Authors: Yikun Li, Eric B. Dammer, Xiaohui Zhang-Brotzge, Scott Chen, Duc M. Duong, Nicholas T. Seyfried et al.
    eNeuro

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Mouse Cystatin C Antibody
By Anonymous on 08/30/2023
Application: Flow Sample Tested: CHO Chinese hamster ovary cell line Species: Mouse