Mouse DAN Antibody

Catalog # Availability Size / Price Qty
MAB7551
MAB7551-SP
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Mouse DAN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DAN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) DAN, recombinant chicken Caronte, rhCerberus, or recombinant mouse Gremlin is observed.
Source
Monoclonal Rat IgG2A Clone # 123202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DAN
Ala17-Asp178
Accession # Q61477.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse DAN (Catalog # 755-DA)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 5-20 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP) to immobilized Recombinant Mouse DAN (Catalog # 755-DA) coated at 2 µg/mL (100 µL/well). At 200 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAN

DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in src-transformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP-2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA. It is likely the various DAN family members and other TGF-beta BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGF-beta superfamily ligands.

References
  1. Massage, J. and Y-G. Chen (2000) Genes & Development 14:627.
  2. Perch, J.J.H. et al. (1999) Develop. Biol. 209:98.
  3. Hsu, D.R. et al. (1998) Mol. Cell. 1:673.
Long Name
Differential Screening-selected Gene Aberrant in Neuroblastoma
Entrez Gene IDs
4681 (Human); 17965 (Mouse)
Alternate Names
DAN domain family member 1; DAN; DAND1; MGC8972; NBL1; neuroblastoma suppressor of tumorigenicity 1; neuroblastoma, suppression of tumorigenicity 1; Protein N03; suppression of tumorigenicity 1; Zinc finger protein DAN

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