Mouse Dkk-3 Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Dkk-3

Dkk-3 (Dickkopf-related protein 3; also REIC) is a 65-70 kDa secreted glycoprotein member of the Dickkopf family of molecules.  It is expressed by multiple cell types, including neurons, endothelial cells, keratinocytes and zona glomerulosa cells of the adrenal.  Dkk-3 is reported to be a positive regulator of Wnt signaling.  It is suggested to bind exclusively to Wnt-inhibiting Kremen proteins, thereby blocking their expression on the cell surface.  This makes LRP available for Wnt binding and signaling.  Dkk-3 is also reported to induce the development of DC from monocytes.  Mature mouse Dkk-3 is 328 amino acids (aa) in length (aa 22-349).  It contains one coiled-coil region (aa 39-84), two consecutive Dkk-type Cys-rich regions (aa 147-195 and 208-284), and one phosphoserine site at Ser298.  Over aa 22-349, mouse shares 83% and 92% aa identity with human and rat Dkk-3, respectively.

Product Datasheets

Related Research Areas

• Negative Regulators of EMT
• New Tools: for Developmental Research
• Wnt Family
• Wnt Inhibitors