Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Dkk-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dkk-3
Met1-Ile349
Accession # Q9QUN9

Specificity

Detects mouse Dkk-3 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dkk-3 is observed and less than 1% cross-reactivity with rhDkk-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Dkk‑3 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Dkk‑3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-3

Dkk-3 (Dickkopf-related protein 3; also REIC) is a 65-70 kDa secreted glycoprotein member of the Dickkopf family of molecules. It is expressed by multiple cell types, including neurons, endothelial cells, keratinocytes and zona glomerulosa cells of the adrenal. Dkk-3 is reported to be a positive regulator of Wnt signaling. It is suggested to bind exclusively to Wnt-inhibiting Kremen proteins, thereby blocking their expression on the cell surface. This makes LRP available for Wnt binding and signaling. Dkk-3 is also reported to induce the development of DC from monocytes. Mature mouse Dkk-3 is 328 amino acids (aa) in length (aa 22-349). It contains one coiled-coil region (aa 39-84), two consecutive Dkk-type Cys-rich regions (aa 147-195 and 208-284), and one phosphoserine site at Ser298. Over aa 22-349, mouse shares 83% and 92% aa identity with human and rat Dkk-3, respectively.

Long Name

Dickkopf-3

Alternate Names

Dkk3, REIC

Entrez Gene IDs

27122 (Human); 50781 (Mouse)

Gene Symbol

DKK3

UniProt

Q9QUN9

Additional Dkk-3 Products

Product Documents for Mouse Dkk‑3 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Dkk‑3 Biotinylated Antibody

For research use only

Citations for Mouse Dkk‑3 Biotinylated Antibody

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