Mouse Dkk‑3 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF948AFP488

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG
View all Dkk-3 Primary Antibodies »

Product Specifications

Specificity

Detects mouse Dkk-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Dkk‑3 is observed and less than 1% cross-reactivity with rhDkk-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Dkk‑3 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Dkk-3

Dkk-3 (Dickkopf-related protein 3; also REIC) is a 65-70 kDa secreted glycoprotein member of the Dickkopf family of molecules.  It is expressed by multiple cell types, including neurons, endothelial cells, keratinocytes and zona glomerulosa cells of the adrenal.  Dkk-3 is reported to be a positive regulator of Wnt signaling.  It is suggested to bind exclusively to Wnt-inhibiting Kremen proteins, thereby blocking their expression on the cell surface.  This makes LRP available for Wnt binding and signaling.  Dkk-3 is also reported to induce the development of DC from monocytes.  Mature mouse Dkk-3 is 328 amino acids (aa) in length (aa 22-349).  It contains one coiled-coil region (aa 39-84), two consecutive Dkk-type Cys-rich regions (aa 147-195 and 208-284), and one phosphoserine site at Ser298.  Over aa 22-349, mouse shares 83% and 92% aa identity with human and rat Dkk-3, respectively.

Long Name

Dickkopf-3

Alternate Names

Dkk3, REIC

Entrez Gene IDs

27122 (Human); 50781 (Mouse)

Gene Symbol

DKK3

UniProt

Q9QUN9

Additional Dkk-3 Products

Product Documents for Mouse Dkk‑3 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Dkk‑3 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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