Mouse Dkk‑3 Antibody

Detection of Mouse Dkk-3 by Western Blot

Over-expression of Dkk3-induced atrophy in cultured myotubes. a Representative images of myotubes over-expressing Dkk3. Primary myotubes were infected by adenovirus-encoding Dkk3 or control vector. Seventy two hours after infection, the myotubes over-expressing Dkk3 were significantly thinner than those infected by control vector. Green indicated myosin heavy chain (MYHC) immunofluorescent staining. Blue indicated DAPI staining of nuclei; Merge indicated merged images of MYHC and DAPI. Scale bars, 50 μm. b Quantification of the average diameters of the myotubes over-expressing Dkk3 or vector control. Error bars indicated s.d. and were based on three independent experiments. The diameters of 900 myotubes were measured in each experiment. ** indicated p < 0.01. cFbxo32 and Trim63 expression levels increased after Dkk3 over-expression. Vector indicated myotubes infected with adenovirus-encoding vector control. Dkk3 indicated myotubes infected with adenovirus-encoding Dkk3. Error bars indicated s.d. and were based on three independent experiments. ** indicated p < 0.01. d Over-expression of Dkk3 increased the protein levels of Fbxo32 and Trim63. Immunoblotting assays were performed with whole-cell extracts from myotubes over-expressing Dkk3 or vector control. Dkk3 was tagged with Flag tag. GAPDH served as internal control. All p-values were based on two-tailed t-test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29717119), licensed under a CC-BY license. Not internally tested by R&D Systems.