Mouse Dkk-3 Antibody Summary
Met1-Ile349
Accession # Q9QUN9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Dkk-3 by Western Blot Over-expression of Dkk3-induced atrophy in cultured myotubes. a Representative images of myotubes over-expressing Dkk3. Primary myotubes were infected by adenovirus-encoding Dkk3 or control vector. Seventy two hours after infection, the myotubes over-expressing Dkk3 were significantly thinner than those infected by control vector. Green indicated myosin heavy chain (MYHC) immunofluorescent staining. Blue indicated DAPI staining of nuclei; Merge indicated merged images of MYHC and DAPI. Scale bars, 50 μm. b Quantification of the average diameters of the myotubes over-expressing Dkk3 or vector control. Error bars indicated s.d. and were based on three independent experiments. The diameters of 900 myotubes were measured in each experiment. ** indicated p < 0.01. cFbxo32 and Trim63 expression levels increased after Dkk3 over-expression. Vector indicated myotubes infected with adenovirus-encoding vector control. Dkk3 indicated myotubes infected with adenovirus-encoding Dkk3. Error bars indicated s.d. and were based on three independent experiments. ** indicated p < 0.01. d Over-expression of Dkk3 increased the protein levels of Fbxo32 and Trim63. Immunoblotting assays were performed with whole-cell extracts from myotubes over-expressing Dkk3 or vector control. Dkk3 was tagged with Flag tag. GAPDH served as internal control. All p-values were based on two-tailed t-test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29717119), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dkk-3
Dkk-3 (Dickkopf-related protein 3; also REIC) is a 65-70 kDa secreted glycoprotein member of the Dickkopf family of molecules. It is expressed by multiple cell types, including neurons, endothelial cells, keratinocytes and zona glomerulosa cells of the adrenal. Dkk-3 is reported to be a positive regulator of Wnt signaling. It is suggested to bind exclusively to Wnt-inhibiting Kremen proteins, thereby blocking their expression on the cell surface. This makes LRP available for Wnt binding and signaling. Dkk-3 is also reported to induce the development of DC from monocytes. Mature mouse Dkk-3 is 328 amino acids (aa) in length (aa 22-349). It contains one coiled-coil region (aa 39-84), two consecutive Dkk-type Cys-rich regions (aa 147-195 and 208-284), and one phosphoserine site at Ser298. Over aa 22-349, mouse shares 83% and 92% aa identity with human and rat Dkk-3, respectively.
Product Datasheets
Citations for Mouse Dkk-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer's disease, restores synapse integrity and memory in a disease mouse model
Authors: Martin Flores, N;Podpolny, M;McLeod, F;Workman, I;Crawford, K;Ivanov, D;Leonenko, G;Escott-Price, V;Salinas, PC;
eLife
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Dynamic Interstitial Cell Response during Myocardial Infarction Predicts Resilience to Rupture in Genetically Diverse Mice
Authors: E Forte, DA Skelly, M Chen, S Daigle, KA Morelli, O Hon, VM Philip, MW Costa, NA Rosenthal, MB Furtado
Cell Rep, 2020-03-03;30(9):3149-3163.e6.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dkk3 dependent transcriptional regulation controls age related skeletal muscle atrophy
Authors: J Yin, L Yang, Y Xie, Y Liu, S Li, W Yang, B Xu, H Ji, L Ding, K Wang, G Li, L Chen, P Hu
Nat Commun, 2018-05-01;9(1):1752.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The effects of Dickkopf-4 on the proliferation, differentiation, and apoptosis of osteoblasts.
Authors: Hiramitsu S, Terauchi M, Kubota T
Endocrinology, 2013-10-08;154(12):4618-26.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The Dickkopf-homolog 3 is expressed in tumor endothelial cells and supports capillary formation.
Authors: Untergasser G, Steurer M, Zimmermann M, Hermann M, Kern J, Amberger A, Gastl G, Gunsilius E
Int. J. Cancer, 2008-04-01;122(7):1539-47.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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