Mouse E-Cadherin APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7481A-100
FAB7481A-025
Detection of E‑Cadherin in D3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Mouse E-Cadherin APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse E-Cadherin in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 114420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
Asp157-Val709 (predicted)
Accession # P09803
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of E-Cadherin antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of E‑Cadherin in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse E-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB7481A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: E-Cadherin

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Mouse E-Cadherin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics
    Authors: C Guo, W Kong, K Kamimoto, GC Rivera-Gon, X Yang, Y Kirita, SA Morris
    Genome Biol., 2019-05-09;20(1):90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The Impact of Microenvironment on the Synovial Sarcoma Transcriptome
    Authors: H Jin, JJ Barrott, MG Cable, MJ Monument, DM Lerman, K Smith-Fry, D Nollner, KB Jones
    Cancer Microenviron, 2017-04-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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