Mouse E-Cadherin Antibody

Catalog # Availability Size / Price Qty
MAB7481
MAB7481-SP
Detection of Mouse E‑Cadherin by Western Blot.
2 Images
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)

Mouse E-Cadherin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse E-Cadherin in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 114420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
Asp157-Val709 (predicted)
Accession # P09803
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
mIMCD‑3 mouse epithelial cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed D3 mouse embryonic stem cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse E-Cadherin antibody by Western Blot. View Larger

Detection of Mouse E‑Cadherin by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse E-Cadherin Monoclonal Antibody (Catalog # MAB7481) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry View Larger

Detection of E‑Cadherin in mlMCD3 cells by Flow Cytometry. mlMCD3 cells were stained with Rat Anti-Mouse E‑Cadherin Monoclonal Antibody (Catalog # MAB7481, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Cadherin

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Mouse E-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. A dietary change to a high-fat diet initiates a rapid adaptation of the intestine
    Authors: JR Enriquez, HA McCauley, KX Zhang, JG Sanchez, GT Kalin, RA Lang, JM Wells
    Cell Reports, 2022-11-15;41(7):111641.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. IL-33 promotes gastric tumour growth in concert with activation and recruitment of inflammatory myeloid cells
    Authors: CP Tran, M Scurr, L O'Connor, JN Buzzelli, GZ Ng, SCN Chin, LA Stamp, T Minamoto, AS Giraud, LM Judd, P Sutton, TR Menheniott
    Oncotarget, 2022-06-01;13(0):785-799.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus
    Authors: PQ Duy, SC Weise, C Marini, XJ Li, D Liang, PJ Dahl, S Ma, A Spajic, W Dong, J Juusola, E Kiziltug, AJ Kundishora, S Koundal, MZ Pedram, LA Torres-Fer, K Händler, E De Domenic, M Becker, T Ulas, SA Juranek, E Cuevas, LT Hao, B Jux, AMM Sousa, F Liu, SK Kim, M Li, Y Yang, Y Takeo, A Duque, C Nelson-Wil, Y Ha, K Selvaganes, SM Robert, AK Singh, G Allington, CG Furey, AT Timberlake, BC Reeves, H Smith, A Dunbar, T DeSpenza, J Goto, A Marlier, A Moreno-De-, X Yu, WE Butler, BS Carter, EMR Lake, RT Constable, P Rakic, H Lin, E Deniz, H Benveniste, NS Malvankar, JI Estrada-Ve, CA Walsh, SL Alper, JL Schultze, K Paeschke, A Doetzlhofe, FG Wulczyn, SC Jin, RP Lifton, N Sestan, W Kolanus, KT Kahle
    Nature Neuroscience, 2022-04-04;25(4):458-473.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Intraepithelial lymphocytes suppress the intestinal tumor growth by cell-to-cell contact via CD103/E-cadherin signal
    Authors: R Morikawa, Y Nemoto, Y Yonemoto, S Tanaka, Y Takei, S Oshima, T Nagaishi, K Tsuchiya, K Nozaki, T Mizutani, T Nakamura, M Watanabe, R Okamoto
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties
    Sci Rep, 2016-11-29;6(0):37914.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation
    J Bone Miner Res, 2016-10-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  7. The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
    Authors: Bittencourt F, Wu S, Bridges J, Miller W
    J Virol, 2014-08-06;88(20):11811-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
    Authors: Zhang, Hanwei, Chin, Arnold I
    PLoS ONE, 2014-04-14;9(4):e94793.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Integrin alpha9beta1 in airway smooth muscle suppresses exaggerated airway narrowing.
    J. Clin. Invest., 2012-07-09;122(8):2916-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
    Authors: Moore RN, Cherry JF, Mathur V
    Stem Cells Dev., 2011-06-01;21(1):30-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Neutralization

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Reviews for Mouse E-Cadherin Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Mouse E-Cadherin Antibody
By Anonymous on 11/11/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: keratinocytes Species: Mouse

Mouse E-Cadherin Antibody
By Anonymous on 02/24/2017
Application: WB Sample Tested: Breast cancer cells Species: Human and Mouse

Comparative expression of mouse E-cadherin in human breast cancer Mary-X spheroids extracted from tumor and the surrounding nu/nu mouse tissue. Dilution: 1:500 in 5% BSA in PBS. Secondary: anti-rat IgG 1:5,000.