Mouse E-Cadherin Antibody
Mouse E-Cadherin Antibody Summary
Asp157-Val709 (predicted)
Accession # P09803
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse E‑Cadherin by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse E-Cadherin Monoclonal Antibody (Catalog # MAB7481) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Cadherin
Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) 251:1451.
Product Datasheets
Citations for Mouse E-Cadherin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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IL-33 promotes gastric tumour growth in concert with activation and recruitment of inflammatory myeloid cells
Authors: CP Tran, M Scurr, L O'Connor, JN Buzzelli, GZ Ng, SCN Chin, LA Stamp, T Minamoto, AS Giraud, LM Judd, P Sutton, TR Menheniott
Oncotarget, 2022;13(0):785-799.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus
Authors: PQ Duy, SC Weise, C Marini, XJ Li, D Liang, PJ Dahl, S Ma, A Spajic, W Dong, J Juusola, E Kiziltug, AJ Kundishora, S Koundal, MZ Pedram, LA Torres-Fer, K Händler, E De Domenic, M Becker, T Ulas, SA Juranek, E Cuevas, LT Hao, B Jux, AMM Sousa, F Liu, SK Kim, M Li, Y Yang, Y Takeo, A Duque, C Nelson-Wil, Y Ha, K Selvaganes, SM Robert, AK Singh, G Allington, CG Furey, AT Timberlake, BC Reeves, H Smith, A Dunbar, T DeSpenza, J Goto, A Marlier, A Moreno-De-, X Yu, WE Butler, BS Carter, EMR Lake, RT Constable, P Rakic, H Lin, E Deniz, H Benveniste, NS Malvankar, JI Estrada-Ve, CA Walsh, SL Alper, JL Schultze, K Paeschke, A Doetzlhofe, FG Wulczyn, SC Jin, RP Lifton, N Sestan, W Kolanus, KT Kahle
Nature Neuroscience, 2022;25(4):458-473.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A dietary change to a high-fat diet initiates a rapid adaptation of the intestine
Authors: JR Enriquez, HA McCauley, KX Zhang, JG Sanchez, GT Kalin, RA Lang, JM Wells
Cell Reports, 2022;41(7):111641.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Intraepithelial lymphocytes suppress the intestinal tumor growth by cell-to-cell contact via CD103/E-cadherin signal
Authors: R Morikawa, Y Nemoto, Y Yonemoto, S Tanaka, Y Takei, S Oshima, T Nagaishi, K Tsuchiya, K Nozaki, T Mizutani, T Nakamura, M Watanabe, R Okamoto
Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation
J Bone Miner Res, 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties
Sci Rep, 2016;6(0):37914.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
Authors: Bittencourt F, Wu S, Bridges J, Miller W
J Virol, 2014;88(20):11811-24.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
Authors: Zhang, Hanwei, Chin, Arnold I
PLoS ONE, 2014;9(4):e94793.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
Authors: Moore RN, Cherry JF, Mathur V
Stem Cells Dev., 2012;21(1):30-41.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Integrin alpha9beta1 in airway smooth muscle suppresses exaggerated airway narrowing.
J. Clin. Invest., 2012;122(8):2916-27.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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Comparative expression of mouse E-cadherin in human breast cancer Mary-X spheroids extracted from tumor and the surrounding nu/nu mouse tissue. Dilution: 1:500 in 5% BSA in PBS. Secondary: anti-rat IgG 1:5,000.
