Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.
Mouse E‑Cadherin PE‑conjugated Antibody
R&D Systems | Catalog # FAB7481P
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 114420
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
Asp157-Val709 (predicted)
Accession # P09803
Asp157-Val709 (predicted)
Accession # P09803
Specificity
Detects mouse E-Cadherin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse E‑Cadherin PE‑conjugated Antibody
Detection of E‑Cadherin in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse E-Cadherin PE-conjugated Monoclonal Antibody (Catalog # FAB7481P, filled histogram) or isotype control antibody (IC006P, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.
Detection of E‑Cadherin in mlMCD3 cells by Flow Cytometry.
mlMCD3 cells were stained with Rat Anti-Mouse E‑Cadherin PE‑conjugated Monoclonal Antibody (Catalog # FAB7481P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse E‑Cadherin PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: D3 mouse embryonic stem cell line stained in buffer containing Ca2+ and Mg2+ and mIMCD‑3 mouse epithelial cells
Sample: D3 mouse embryonic stem cell line stained in buffer containing Ca2+ and Mg2+ and mIMCD‑3 mouse epithelial cells
Reviewed Applications
Read 3 reviews rated 4.3 using FAB7481P in the following applications:
Spectra Viewer
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Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: E-Cadherin
References
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) 251:1451.
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
UniProt
Additional E-Cadherin Products
Product Documents for Mouse E‑Cadherin PE‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse E‑Cadherin PE‑conjugated Antibody
For research use only
Citations for Mouse E‑Cadherin PE‑conjugated Antibody
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Customer Reviews for Mouse E‑Cadherin PE‑conjugated Antibody (3)
4.3 out of 5
3 Customer Ratings
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Application: Immunohistochemistry-FrozenSample Tested: Lung tissueSpecies: MouseVerified Customer | Posted 04/16/2018Red: E-Cadherin Blue: DAPI+ nuclei
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Application: Flow CytometrySample Tested: Lung tissueSpecies: MouseVerified Customer | Posted 03/20/2018Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets. The single cell suspension was isolated from lung of WT B6 mouse and stained with the E-Cadherin PE-conjugated antibody(1:100) for 30 minutes. Cells were analyzed by flow cytometer.
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Application: Flow CytometrySample Tested: Primary mouse lung microvascular endothelial cellSpecies: MouseVerified Customer | Posted 09/08/2017The antibody was diluted at 1:100 with FACS buffer.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
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- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
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- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
