Mouse E-Cadherin PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7481P-100
FAB7481P-025
Detection of E‑Cadherin in D3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (3)
FAQs
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Mouse E-Cadherin PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse E-Cadherin in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 114420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
Asp157-Val709 (predicted)
Accession # P09803
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of E-Cadherin antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of E‑Cadherin in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse E-Cadherin PE-conjugated Monoclonal Antibody (Catalog # FAB7481P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: E-Cadherin

Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Mouse E-Cadherin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. M1-Polarized Macrophages Promote Self-Renewing Phenotype of Hepatic Progenitor Cells with Jagged1-Notch Signalling Involved: Relevance in Primary Sclerosing Cholangitis
    Authors: H Li, S Sun, Q Lei, P Lei, X Cai, C Wan, G Shen
    J Immunol Res, 2018;2018(0):4807145.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A Transcriptional switch point during hematopoietic stem and progenitor cell ontogeny
    Stem Cells Dev., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Adenovirus-mediated intratumoral expression of immunostimulatory proteins in combination with systemic Treg inactivation induces tumor-destructive immune responses in mouse models.
    Authors: Liu Y, Tuve S, Persson J, Beyer I, Yumul R, Li ZY, Tragoolpua K, Hellstrom KE, Roffler S, Lieber A
    Cancer Gene Ther., 2011;18(6):407-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse E-Cadherin PE-conjugated Antibody
By Jim Hsiao on 03/20/2018
Application: Flow Sample Tested: Lung tissue Species: Mouse

Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets.

The single cell suspension was isolated from lung of WT B6 mouse and stained with the E-Cadherin PE-conjugated antibody(1:100) for 30 minutes. Cells were analyzed by flow cytometer.