E-Selectin, also known as CD62E, is expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. It is a lectin that binds to sialylated or fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells. E-Selectin mediates the initial attachment of circulating leukocytes to the blood vessel wall during inflammation. This interaction permits leukocytes to roll along the vascular endothelium. Subsequent ICAM-1 and VCAM-1 mediated adhesion leads to extravasation of the leukocyte through the blood vessel wall into the extracellular matrix tissue.
Mouse E-Selectin/CD62E Quantikine ELISA Kit
R&D Systems | Catalog # MES00
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
4.7 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
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Product Summary for Mouse E-Selectin/CD62E Quantikine ELISA Kit
The Quantikine Mouse soluble E-Selectin (sE-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sE-selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sE-selectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sE-selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sE-selectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse E-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 52 | 152 | 464 | 49 | 144 | 440 |
| Standard Deviation | 4.6 | 9.5 | 41 | 4.3 | 10 | 29 |
| CV% | 8.8 | 6.3 | 8.8 | 8.8 | 6.9 | 6.6 |
Recovery for Mouse E-Selectin/CD62E Quantikine ELISA Kit
The recovery of mouse sE-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 95 | 83-102 |
| EDTA Plasma (n=4) | 101 | 78-120 |
| Serum (n=10) | 98 | 83-118 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with high concentrations of mouse sE-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse E-Selectin/CD62E Quantikine ELISA Kit
Mouse E-Selectin/CD62E ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: E-Selectin/CD62E
Alternate Names
CD62E, ELAM1, LECAM2, SELE
Gene Symbol
SELE
Additional E-Selectin/CD62E Products
Product Documents for Mouse E-Selectin/CD62E Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse E-Selectin/CD62E Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse E-Selectin/CD62E Quantikine ELISA Kit
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Protocols
View specific protocols for Mouse E-Selectin/CD62E Quantikine ELISA Kit (MES00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
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