Mouse Endocan/ESM-1 Antibody

Catalog # Availability Size / Price Qty
MAB1999
MAB1999-SP
Cell Adhesion Mediated by Endocan/ESM‑1 and Neutral-ization by Mouse Endocan/ ESM‑1 Antibody.
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Mouse Endocan/ESM-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endocan/ESM‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Endocan is observed.
Source
Monoclonal Rat IgG2B Clone # 220008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endocan/ESM‑1
Trp20-Arg184
Accession # Q9QYY7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Endocan/ESM‑1 (Catalog # 1999‑EC) under non-reducing conditions only

Mouse Endocan/ESM-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Endocan/ESM‑1 Biotinylated Antibody (Catalog # BAF1999)

Standard: Recombinant Mouse Endocan Protein, CF (Catalog # 1999-EC)

Neutralization
Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.75-3.5 µg/mL in the presence of 25 µg/mL Recombinant Mouse Endocan/ESM‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Adhesion Mediated by Endocan/ESM‑1 and Neutral-ization by Mouse Endocan/ ESM‑1 Antibody. View Larger

Cell Adhesion Mediated by Endocan/ESM‑1 and Neutral-ization by Mouse Endocan/ ESM‑1 Antibody. Recombinant Mouse Endocan/ESM-1 (Catalog # 1999-EC), immo-bilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM-1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Endocan/ESM-1 Monoclonal Antibody (Catalog # MAB1999). The ND50 is typically 0.75-3.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endocan/ESM-1

Endocan (endothelial cell proteoglycan), also known as endothelial-cell specific molecule-1 (ESM-1), is a 50 kDa monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan shares 96% and 74% aa identity with rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa 82-131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF-alpha and VEGF, (1, 7) and is known to bind to LFA-1 (integrin alpha L beta 2) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).

References
  1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
  2. Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
  3. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
  4. Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
  5. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
  6. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
  7. Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.
Entrez Gene IDs
11082 (Human); 71690 (Mouse)
Alternate Names
Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6

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