Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all ENPP-7/Alk-SMase Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ENPP‑7/Alk‑SMase
Ala22-Gln421
Accession # NP_001025462

Specificity

Detects mouse ENPP‑7/Alk‑SMase in Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse ENPP‑7/Alk‑SMase Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse ENPP-7/Alk-SMase (Catalog # 5160-EN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ENPP-7/Alk-SMase

ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30 - 36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).

References

  1. Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
  2. Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
  3. Wu, J. et al. (2006) Biochem J. 394:299.
  4. Hertervig, E. et al. (1996) Cancer. 79:448.
  5. Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Alternate Names

Alk-SMase, ENPP7, NPP7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Gene Symbol

ENPP7

UniProt

NP_001025462

Additional ENPP-7/Alk-SMase Products

Product Documents for Mouse ENPP‑7/Alk‑SMase Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ENPP‑7/Alk‑SMase Biotinylated Antibody

For research use only

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