ENPP‑7/Alk-SMase in intestine.
ENPP‑7/Alk-SMase was detected in perfusion fixed frozen sections of mouse intestine using Mouse ENPP‑7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse ENPP‑7/Alk-SMase by Western Blot.
Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse ENPP‑7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ENPP‑7/Alk-SMase at approximately 60kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30-36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).
Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
Wu, J. et al. (2006) Biochem J. 394:299.
Hertervig, E. et al. (1996) Cancer. 79:448.
Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.
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